| Title | Anti-cancer activity of nitrones in the Apc(Min/+) model of colorectal cancer. | | Author(s) | Floyd RA, Towner RA, Wu D, Abbott A, Cranford R, Branch D, Guo WX, Foster SB, Jones I, Alam R, Moore D, Allen T, Huycke M | | Institution | Experimental Therapeutics Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. | | Source | Free Radic Res 2009 Nov 3. | | Abstract | Abstract The nitrones of alpha-phenyl-tert-butyl nitrone (PBN) and 4-hydroxyl-PBN (4-OH-PBN) that have anti-cancer activity in models of liver cancer and glioblastomas were tested in the ApcMin/+ mouse model. Mice were administered PBN and 4-OH-PBN in drinking water and intestinal tumour size and number assessed after 3-4 months. Throughout the experiment, contrast-enhanced magnetic resonance imaging (MRI) was used to monitor colon tumours. MRI data showed a time-dependent significant increase in total colonic signal intensity in sham-treated mice, but a significant decrease for PBN-treated mice and slight decrease for 4-OHPBN treated mice, probably due to the limited water solubility of 4-OH-PBN. Final pathological and percentage survival data agreed with the MRI data. PBN had little effect on oxaliplatin-mediated killing of HCT116 colon cancer cells and caused only a slight decrease in the amount of active fraction caspase 3 in oxaliplatin-treated cells. PBN has significant anti-cancer activity in this model of intestinal neoplasia. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19886748 |
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