| Title | Small Intestinal Submucosa Gel as a Potential Scaffolding Material for Cardiac Tissue Engineering. | | Author(s) | Crapo PM, Wang Y | | Institution | Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Atlanta, GA 30332, USA. | | Source | Acta Biomater 2009 Oct 31. | | Abstract | Cardiac tissue engineering typically utilizes protein-rich scaffolding materials and growth factors to improve cardiac tissue function in vitro and in vivo. The objectives of this preliminary study were (I) to investigate the potential of porcine small intestinal submucosa gel (SIS gel) in cardiac tissue engineering and (II) to compare the function of tissue based on either SIS gel or Matrigel, a tumor-derived benchmark material. Neonatal rat cardiac cells were combined with either SIS gel or Matrigel and cultured on porous elastomeric scaffolds composed of poly(glycerol sebacate) for 13 days. Tissue function was assessed by measuring contraction rates twice daily. Tissue morphology was compared qualitatively by H&E staining. Normalized troponin T expression (troponin T:DNA) was compared using image analysis. SIS gel constructs contracted at significantly higher rates than Matrigel constructs on days 8-11. Normalized troponin T expression was significantly higher in SIS gel constructs compared to Matrigel constructs. In summary, this research demonstrates that: (I) SIS gel can be used to create contractile engineered cardiac tissue; and (II) SIS gel produced engineered cardiac tissues with a more physiologic contraction rate and higher phenotypic protein expression based on basic in vitro examinations performed in this study. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19887120 |
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