Clinical Itraconazole-Resistant Strains of Aspergillus fumigatus, Isolated Serially from a Lung Aspergilloma Patient with Pulmonary Tuberculosis, can be Detected with Real-Time PCR Method. Mycopathologia [Mycopathologia] Journal article | | Title | Clinical Itraconazole-Resistant Strains of Aspergillus fumigatus, Isolated Serially from a Lung Aspergilloma Patient with Pulmonary Tuberculosis, can be Detected with Real-Time PCR Method. | | Author(s) | Xu H, Chen W, Li L, Wan Z, Li R, Liu W | | Institution | Department of Dermatology, Peking University First Hospital, and Research Center for Medical Mycology, Peking University, No. 8 Xishiku St., 100034, Xicheng District, Beijing, China. | | Source | Mycopathologia 2009 Nov 4. | | Abstract | The invasive aspergillosis, which is commonly caused by Aspergillus fumigatus (A. fumigatus), has increased in recent years. Traditional methods for finding out antifungal resistant strains would take more than 2 weeks, which cannot satisfy the needs of rapid detection. In this study, a real-time PCR method for detection of the serial itraconazole-resistant strains of A. fumigatus isolated from a lung aspergilloma patient was developed. The results showed that the TacMAN-MGB probes, which were covered the loci Gly54, Leu98, Gly138, and Met220 of the enzyme CYP51A coded by the gene cyp51A, as well as the 34-bp tandem repeated sequence in the promoter region (-288 and -322 from the start codon) of this gene, could detect the serial itraconazole-resistant strains of A. fumigatus in our study. Besides, this method takes just 6 h to complete the whole detection. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19888672 |
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