Characterising the post-antifungal effects of micafungin against Candida albicans, Candida glabrata, Candida parapsilosis and Candida krusei isolates. International journal of antimicrobial agents [Int J Antimicrob Agents] Journal article

Title	Characterising the post-antifungal effects of micafungin against Candida albicans, Candida glabrata, Candida parapsilosis and Candida krusei isolates.
Author(s)	Nguyen KT, Ta P, Hoang BT, Cheng S, Hao B, Nguyen MH, Clancy CJ
Institution	Department of Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
Source	Int J Antimicrob Agents 2009 Nov 2.
Abstract	In this study, we measured time-kills and post-antifungal effects (PAFEs) for micafungin against Candida albicans (n=4), Candida glabrata (n=3), Candida parapsilosis (n=3) and Candida krusei (n=2) isolates and further characterised the PAFEs. Minimum inhibitory concentrations (MICs) were 0.5-1.0mg/L against C. parapsilosis and 0.008-0.125mg/L against the other species. Micafungin caused kills >1log at 1xMIC, 4xMIC (range 1.19-3.10log) and 16xMIC (2.27-3.68log), achieving fungicidal levels (>/=3log) against nine isolates. One-hour drug exposure during PAFE experiments resulted in kills of 0.73-2.88log and 1.72-3.55log at 4x and 16x MIC, respectively, achieving fungicidal levels against five isolates. Isolates of each species collected 8h after a 1-h exposure to micafungin (4x and 16x MIC) were hypersusceptible to sodium dodecyl sulphate (SDS) and Calcofluor White. Cells tested during the PAFE period demonstrated cell wall disturbances as evident on electron micrographs as well as significant reductions in adherence to epithelial cells. Phagocytosis by J774 macrophages was significantly enhanced for three PAFE isolates tested. Micafungin is fungicidal and exerts PAFEs that kill diverse Candida spp., disturb cell walls of viable organisms, reduce adherence and enhance susceptibility to phagocytosis.
Language	ENG
Pub Type(s)	JOURNAL ARTICLE
PubMed ID	19889519

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