| Title | Autocrine and paracrine roles for ATP and serotonin in mouse taste buds. | | Author(s) | Huang YA, Dando R, Roper SD | | Institution | Department of Physiology and Biophysics and Program in Neuroscience, University of Miami School of Medicine, Miami, FL 33136, USA. | | Source | J Neurosci 2009 Nov 4; 29(44):13909-18. | | MeSH | Adenosine Triphosphate
Animals
Autocrine Communication
CHO Cells
Cricetinae
Cricetulus
Female
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Paracrine Communication
Serotonin
Taste
Taste Buds
| | Abstract | Receptor (type II) taste bud cells secrete ATP during taste stimulation. In turn, ATP activates adjacent presynaptic (type III) cells to release serotonin (5-hydroxytryptamine, or 5-HT) and norepinephrine (NE). The roles of these neurotransmitters in taste buds have not been fully elucidated. Here we tested whether ATP or 5-HT exert feedback onto receptor (type II) cells during taste stimulation. Our previous studies showed NE does not appear to act on adjacent taste bud cells, or at least on receptor cells. Our data show that 5-HT released from presynaptic (type III) cells provides negative paracrine feedback onto receptor cells by activating 5-HT(1A) receptors, inhibiting taste-evoked Ca(2+) mobilization in receptor cells, and reducing ATP secretion. The findings also demonstrate that ATP exerts positive autocrine feedback onto receptor (type II) cells by activating P2Y1 receptors and enhancing ATP secretion. These results begin to sort out how purinergic and aminergic transmitters function within the taste bud to modulate gustatory signaling in these peripheral sensory organs. | | Language | eng | | Pub Type(s) | Comparative Study
Journal Article
Research Support, N.I.H., Extramural
| | PubMed ID | 19890001 |
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