| Title | Development and Validation of a SYBR-Green I Real-Time PCR Protocol To Detect Hazelnut ( Corylus avellana L.) in Foods through Calibration via Plasmid Reference Standard. | | Author(s) | D'Andrea M, Coïsson JD, Travaglia F, Garino C, Arlorio M | | Institution | DISCAFF and DFB Center, Universita del Piemonte Orientale "A. Avogadro", via Bovio 6, 28100 Novara, Italy. | | Source | J Agric Food Chem 2009 Nov 5. | | Abstract | Many tree nuts are considered to be a serious problem in food safety, because of the presence of causative factors in IgE-mediated food allergies. Among these, hazelnut ( Corylus avellana L.) seeds are largely used in a range of confectionery products and contain many well-characterized allergens. DNA-based methods and ELISA tests may prove to be useful to assess the presence of hidden ingredients in foods. The aim of this work was the development and validation of a species-specific SYBR Green I real-time PCR protocol for the detection of hazelnut in foods. A novel efficient primer pair on the Cor a 8 genomic coding region was designed by preparing a plasmid vector-based internal reference standard to calibrate the PCR. A good sensitivity, down to 20 (genomic) and 15 (plasmid) DNA copies, was established. All of the commercial samples considered in our study (containing hazelnut as ingredient or as a potential trace cross-contamination) were effectively amplified by PCR, showing a perfect correspondence with an ELISA commercial test, employed as a reference standard method. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19891480 |
|
