Kumagai M, Inaba T, Makioka A, Ishiwata K, Onishi K, Watanabe N
An Improved Glycerin Jelly Mounting Procedure for Permanent Preparations of Helminth Eggs. [JOURNAL ARTICLE]
J Parasitol 2009 Nov 5.:1.
Many attempts have been undertaken to make permanent preparations of helminth eggs. However, none was both durable itself and free from deforming of thin-shell eggs, such as hookworm eggs. To overcome these drawbacks, we have modified the glycerin jelly mounting procedure in 2 aspects. First, we gradually changed the media in which the helminth eggs soaked, from 10% formalin via water to the 70% ethanol and 5% glycerin solution. It took 10 days, which should be much longer than the processes previously reported. Second, we used a hole slide glass instead of a slide glass. The eggs of 11 species of helminths prepared by this procedure have kept their morphology without apparent change for more than 4 yr.
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