The effect of glucocorticoids on ERK1/2 phosphorylation during maturation of lamb oocytes and their subsequent fertilization and cleavage ability in vitro. Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] Journal article | | Title | The effect of glucocorticoids on ERK1/2 phosphorylation during maturation of lamb oocytes and their subsequent fertilization and cleavage ability in vitro. | | Author(s) | González R, Ruiz-León Y, Gomendio M, Roldan ER | | Institution | Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales (CSIC), 28006-Madrid, Spain. | | Source | Reprod Toxicol 2009 Nov 2. | | Abstract | High levels of glucocorticoids may alter reproduction, but little is known about their direct actions on oocyte maturation, fertilization and subsequent development. Earlier work suggested negative effects of cortisol or dexamethasone on oocyte maturation but differences were noted between animal models. Both glucocorticoids reduce the p34(cdc2)-cyclin B1 complex but it is unknown if other signaling pathways important for meiosis progression are affected. In this study, using sheep oocytes as a model system, we assessed in vitro the effects of increasing concentration of glucocorticoids (0-250muM) on oocyte maturation and underlying changes in the MAP kinase pathway, and the ability of oocytes to undergo fertilization and embryo development. Cortisol decreased oocyte maturation but only at the highest concentration, whereas dexamethasone had no effect. Fertilization and cleavage were not affected. On the other hand, both cortisol and dexamethasone inhibited ERK-1/2 activation in a concentration-dependent manner. It thus seems that oocytes can overcome deleterious effects of glucocorticoids during maturation despite the decrease in ERK-1/2 activity, but repercussions in vivo should be further explored. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19892010 |
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