M Cell Targeted Ocular Immunization - Effect on Immunoglobulins in Tears, Feces, and Serum. Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] Journal article | | Title | M Cell Targeted Ocular Immunization - Effect on Immunoglobulins in Tears, Feces, and Serum. | | Author(s) | Phillips TE, Sharp J, Rodgers K, Liu H | | Institution | Biological Sciences, University of Missouri, Columbia, United States. | | Source | Invest Ophthalmol Vis Sci 2009 Nov 5. | | Abstract | PURPOSE: This study investigates whether antigen-sampling M cells, present in the follicle-associated epithelium (FAE) above organized conjunctiva-associated lymphoid tissue in rabbits, bind and retro-transport secretory IgA (sIgA) from the tear film. The hypothesis that IgA-mediated uptake of antigens promotes local and systemic production of immunoglobulins was tested.
METHODS: sIgA binding and retro-translocation by M cells was characterized by immunocytochemistry. Immunoglobulin concentrations in tears, feces and serum were measured using enzyme-linked immunoassays (ELISA) following topical and systemic immunization with either goat IgG anti-rabbit IgA or non-specific goat IgG.
RESULTS: Endogenous sIgA was found associated with the apical membrane of conjunctival M cells. Exogenous anti-IgA immunoglobulins were translocated across M cells. Significant levels of sIgA against goat IgG were present in tears of pre-immune animals. Topical application of either goat IgG specific for rabbit IgA or non-specific goat IgG led to similar increases in antigen-specific IgA in tear, feces, and serum. The antigen-specific IgG response in tears mirrored the serum response for both immunogens consistent with transudation of this immunoglobulin. The IgM response in tears and serum was weak for both immunogens. Systemic immunization did not sustain or enhance the local mucosal IgA responses.
CONCLUSIONS: Conjunctival M cells bind and translocate sIgA from the tear film. Topical conjunctival immunization leads to generation of antigen-specific immunoglobulins from both local and distant mucosae and in serum. Natural antibodies, present in the tear film prior to immunization, may have contributed to similar immune responses to goat anti-rabbit IgA and non-specific goat IgG. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19892871 |
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