| Title | 2-D mapping of skin chromophores in the spectral range 500 - 700 nm. | | Author(s) | Jakovels D, Spigulis J | | Institution | Bio-Optics and Fibre Optics Laboratory, Institute of Atomic Physics and Spectroscopy, University of Latvia, Raina Blvd. 19, LV - 1586, Riga, Latvia. | | Source | J Biophotonics 2009 Nov 5. | | Abstract | The multi-spectral imaging technique has been used for distant mapping of in-vivo skin chromophores by analyzing spectral data at each reflected image pixel and constructing 2-D maps of the relative concentrations of oxy-/deoxy-haemoglobin and melanin. Instead of using a broad visible-NIR spectral range, this study focuses on narrowed spectral band 500-700 nm, speeding-up the signal processing procedure. Regression analysis confirmed that superposition of three Gaussians is optimal analytic approximation for the oxy-haemoglobin absorption tabular spectrum in this spectral band, while superposition of two Gaussians fits well for deoxy-haemoglobin absorption and exponential function - for melanin absorption. The proposed approach was clinically tested for three types of in-vivo skin provocations: ultraviolet irradiance, chemical reaction with vinegar essence and finger arterial occlusion. Spectral range 500-700 nm provided better sensitivity to oxy-haemoglobin changes and higher response stability to melanin than two reduced ranges 500-600 nm and 530-620 nm. ((c) 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19894217 |
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