Xu Q, Wei F, Wang Z, Yang Q, Zhao YD, Chen H
In vivo monitor oxidative burst induced by Cd(2+) stress for the oilseed rape (Brassica napus L.) based on electrochemical microbiosensor. [JOURNAL ARTICLE]
Phytochem Anal 2009 Nov 11.
Introduction - Since the mechanism of Cd(2+) stress for plants is not clear, an in vivo method to monitor Cd(2+) stress for plants is necessary. However, oxidative burst (OB) is a signal messenger in the process of Cd(2+) stress for plants.Objective - To establish an electrochemical method with poly-o-phenylenediamine and Pt microparticle modified Pt electrode (POPD-Pt-MP-Pt) as a microbiosensor for the in vivo detection of oxidative burst induced by Cd(2+) stress in oilseed rape (Brassica napus L.).Methodology - The optimal fabrication of POPD-Pt-MP-Pt biosensor was achieved. Electrochemical signal was collected by amperometry.Results - After oilseed rape was exposed to 84.9 mM CdCl(2) stress, three oxidative bursts were observed in oilseed rape by amperometry at 3.3 h, 8.4 h and 13.2 h, respectively. However, there was no obvious signal observed in the controlled assay.Conclusion - This contribution presents the in vivo monitoring of the OB process induced by Cd(2+) stress in oilseed rape by POPD-Pt-MP-Pt microbiosensor in real-time. The novel electrochemical microbiosensor not only facilitates the real-time study in plant self-defence response to the adverse environment such as Cd(2+) stress, but also provides an effective tool for probing the self-defence mechanism in plants. Copyright (c) 2009 John Wiley & Sons, Ltd.
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