| Title | Quantum Dots Enhanced Ultrasensitive Detection of DNA Adducts. | | Author(s) | Wang Z, Lu M, Wang X, Yin R, Song Y, Le XC, Wang H | | Institution | State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, China, and Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, T6G 2G3, AB, Canada. | | Source | Anal Chem 2009 Nov 19. | | Abstract | Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)-DNA adducts from sensitivity to separation. We for the first time show that the target QD-antibody-DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 x 10(-21) mol in mass and 120 fM in concentration are achieved for BPDE-DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19925008 |
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