| Title | Molecular detection and typing of dengue viruses from archived tissues of fatal cases by rt-PCR and sequencing: diagnostic and epidemiologic implications. | | Author(s) | Bhatnagar J, Blau DM, Shieh WJ, Paddock CD, Drew C, Liu L, Jones T, Patel M, Zaki SR | | Institution | Infectious Diseases Pathology Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. JBhatnagar@cdc.gov | | Source | Am J Trop Med Hyg 2012 Feb; 86(2):335-40. | | MeSH | Adolescent
Adult
Aged
Child
Child, Preschool
Dengue
Dengue Virus
Female
Fixatives
Formaldehyde
Humans
Immunohistochemistry
Infant
Male
Middle Aged
Paraffin Embedding
Prevalence
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, RNA
Serotyping
Specimen Handling
Tissue Fixation
Young Adult
| | Abstract | Diagnosis of dengue virus (DENV) infection in fatal cases is challenging because of the frequent unavailability of blood or fresh tissues. For formalin-fixed, paraffin-embedded (FFPE) tissues immunohistochemistry (IHC) can be used; however, it may not be as sensitive and serotyping is not possible. The application of reverse transcription-polymerase chain reaction (RT-PCR) for the detection of DENV in FFPE tissues has been very limited. We evaluated FFPE autopsy tissues of 122 patients with suspected DENV infection by flavivirus and DENV RT-PCR, sequencing, and DENV IHC. The DENV was detected in 61 (50%) cases by RT-PCR or IHC. The RT-PCR and sequencing detected DENV in 60 (49%) cases (DENV-1 in 16, DENV-2 in 27, DENV-3 in 8, and DENV-4 in 6 cases). No serotype could be identified in three cases. The IHC detected DENV antigens in 50 (40%) cases. The RT-PCR using FFPE tissue improves detection of DENV in fatal cases and provides sequence information useful for typing and epidemiologic studies. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 22302871 |
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