Isolation of human complement factors C3, C5 and H. Journal of immunological methods [J Immunol Methods] Journal article

Title	Isolation of human complement factors C3, C5 and H.
Author(s)	Lundwall A, Eggertsen G
Source	J Immunol Methods 1985 Jul 16; 81(1):147-60.
MeSH	Chromatography, Gel Chromatography, Ion Exchange Complement C3 Complement C3b Inactivator Proteins Complement C5 Complement Factor H Humans Peptide Fragments
Abstract	An improved method for simultaneous purification of complement factors C3, C5 and H from human plasma has been developed. Using an initial batch separation technique with QAE-Sephadex, followed by chromatography on SP-Sephadex and gel filtration in Sephadex G-200, 600 mg of highly pure C3 can be prepared from 1600 ml of plasma. Simultaneously about 70 mg of highly pure factor H and 30 mg of C5 are obtained by chromatography of post SP-Sephadex material on DEAE-Sephacel. A small amount of C3 in the C5 pool is removed by anti-C3-Sepharose. By maleylation or citraconylation of reduced and alkylated C3, the constitutive polypeptide chains are modified in a way that made them separable by ion exchange chromatography.
Language	eng
Pub Type(s)	Journal Article Research Support, Non-U.S. Gov't
PubMed ID	3160789

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