| Title | A new model of embolic stroke produced by photochemical injury to the carotid artery in the rat. | | Author(s) | Futrell N, Watson BD, Dietrich WD, Prado R, Millikan C, Ginsberg MD | | Institution | Cerebral Vascular Disease Research Center, University of Miami School of Medicine, FL 33101. | | Source | Ann Neurol 1988 Mar; 23(3):251-7. | | MeSH | Animals
Carotid Arteries
Carotid Artery Thrombosis
Cerebral Infarction
Disease Models, Animal
Endothelium, Vascular
Intracranial Embolism and Thrombosis
Male
Microscopy, Electron, Scanning
Platelet Aggregation
Rats
Rats, Inbred Strains
| | Abstract | We report a new model of embolic stroke in the rat, based on endothelial disruption and platelet aggregation in the carotid artery, which leads to distal embolization and focal brain infarction. The common carotid artery was irradiated for 6.5 minutes with the focused beam of an argon laser operated at a wavelength of 514.5 nm with a peak power of 2 W and an average power of 400 mw. Ipsilateral cerebral infarcts, ranging in size from 0.1 to 1.7 mm, were produced by platelet emboli in 12 of 13 rats. A total of 44 infarcts were observed in the 12 rats: 29 in the cortex, 6 in the hippocampus, 5 in the thalamus, and 4 in the basal ganglia. Scanning electron microscopy identified platelet aggregates in the carotid artery and in a deep cortical arteriole 50 minutes after the photochemical lesion. Twenty-four hours after the experiment, scanning electron microscopy of the carotid artery revealed damaged endothelium but few remaining adherent platelets. More intense laser irradiation in 8 rats, leading to carotid occlusion, produced an infarct in only 1. This new model can be used to study the acute and chronic pathological changes in the brain associated with platelet embolism. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
| | PubMed ID | 3377448 |
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