Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis. The British journal of venereal diseases. [Br J Vener Dis] Journal article

Title	Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis.
Author(s)	Lindenschmidt EG, Laufs R, Müller F
Source	Br J Vener Dis 1983 Jun; 59(3):151-6.
MeSH	Antibodies, Bacterial Antigens, Bacterial Comparative Study Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Humans Immunoglobulin M Syphilis Syphilis Serodiagnosis Treponema pallidum
Abstract	An enzyme-linked immunosorbent assay (ELISA) using a highly purified soluble antigen prepared from pathogenic Treponema pallidum (Nichols) was carried out to detect specific IgM antibodies in syphilis. Serum specimens from 365 patients with untreated or treated syphilis were tested by the ELISA and by the 19S(IgM) fluorescent treponemal antibody-absorbed test. In the ELISA sera were preabsorbed with aggregated human IgG to eliminate any rheumatoid factor present. Sera from 331 healthy blood donors served as controls. The results of both tests correlated well. The specificity and sensitivity of the treponemal ELISA were both greater than 97%. The assay was easy to perform and may be automated.
Language	eng
Pub Type(s)	Journal Article
PubMed ID	6342706

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