| Title | Characterization of the superoxide-generating system in human peripheral lymphocytes and lymphoid cell lines. | | Author(s) | Kobayashi S, Imajoh-Ohmi S, Kuribayashi F, Nunoi H, Nakamura M, Kanegasaki S | | Institution | Institute of Medical Science, University of Tokyo. | | Source | J Biochem 1995 Apr; 117(4):758-65. | | MeSH | Antigens, Surface
B-Lymphocytes
Blood Proteins
Cell Line
Cell Line, Transformed
Cell Transformation, Viral
Cytochrome b Group
Cytosol
Flow Cytometry
Herpesvirus 4, Human
Humans
Immunoblotting
Lymphoma, T-Cell
Macromolecular Substances
NADPH Oxidase
Neutrophils
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Superoxides
T-Lymphocytes
Tumor Cells, Cultured
| | Abstract | In B lymphocytes, but not T lymphocytes, isolated from human peripheral blood, we detected the four protein components essential for "the respiratory burst" by immunoblot analyses using peptide-directed antibodies. These are two membrane proteins, namely, 91- and 22-kDa subunits of cytochrome b558, and two cytosolic proteins with molecular masses of 47 and 65 kDa. Like in neutrophils, cytochrome b558 was expressed on the cell surface of peripheral B lymphocytes. Mean amounts (n = 8) of the 91-, 22-, 47-, and 65-kDa proteins, respectively, in peripheral B lymphocytes calculated from intensity of the blots were 0.011 +/- 0.003, 0.026 +/- 0.006, 0.179 +/- 0.022, and 0.039 +/- 0.013 relative to those in neutrophils on the basis of cell number. Epstein-Barr virus (EBV)-transformed cell lines derived from normal B lymphocytes and some B cell lines also possessed cytochrome b558 and two cytosolic proteins. Isolated human peripheral B lymphocytes generated the superoxide anion upon cross-linking of surface antigens such as IgM, IgD, IgG, HLA-DR, and CD19. EBV-transformants derived from normal peripheral B lymphocytes and B lymphoid cell lines also generated the superoxide anion when stimulated with various antibodies against surface antigens. These results indicate that peripheral B lymphocytes have substantial amounts of a superoxide-generating system identical to that in phagocytes and that the system is stimulated to generate the superoxide anion by the cross-linking of clonally expressed surface immunoglobulins or of certain surface antigens. | | Language | eng | | Pub Type(s) | Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 7592536 |
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