| Title | The fibrinolytic system in the hemolytic uremic syndrome: in vivo and in vitro studies. | | Author(s) | van de Kar NC, van Hinsbergh VW, Brommer EJ, Monnens LA | | Institution | Gaubius Laboratory, IVVO-TNO, Leiden, The Netherlands. | | Source | Pediatr Res 1994 Aug; 36(2):257-64. | | MeSH | Bacterial Toxins
Cells, Cultured
Child, Preschool
Deamino Arginine Vasopressin
Endothelium, Vascular
Female
Fibrin Fibrinogen Degradation Products
Fibrinolysis
Hemolytic-Uremic Syndrome
Humans
In Vitro
Infant
Male
Plasminogen Activator Inhibitor 1
Research Support, Non-U.S. Gov't
Shiga-Like Toxin I
Tissue Plasminogen Activator
Tumor Necrosis Factor-alpha
von Willebrand Factor
| | Abstract | Fibrinolytic parameters and von Willebrand factor (vWF) antigen were measured in the plasma of 10 patients with hemolytic uremic syndrome (HUS). Samples were taken at presentation and again 2 wk later, before and after infusion of 1-desamino-8-arginine vasopressin. Compared with the plasma values of healthy control children, levels of tissue-plasminogen activator (t-PA) antigen, plasminogen activator inhibitor type I (PAI-1) activity, and vWF as well as fibrin(ogen) degradation products were significantly elevated in the plasma of HUS patients on admission. No response of the fibrinolytic parameters and vWF were seen when 1-desamino-8-arginine vasopressin infusion was given on admission. After 2 wk, t-PA antigen and vWF had partially returned to basal values, and t-PA antigen increased rapidly again after 1-desamino-8-arginine vasopressin infusion. To investigate whether verocytotoxin contributes to the alteration of the fibrinolytic system found in HUS patients, purified verocytotoxin-1 (VT-1) was added to the media of cultured human endothelial cells. Addition of VT-1 alone did not change the production of t-PA, plasminogen activator inhibitor type I, and vWF antigen in these cells. However, when the endothelial cells were preincubated with tumor necrosis factor-alpha to increase the number of VT-1 receptors, VT-1 induced a marked decrease of the synthesis of t-PA, plasminogen activator inhibitor type I, and vWF. This was caused by a decrease in overall protein synthesis in the tumor necrosis factor-alpha- and VT-1-treated endothelial cells.(ABSTRACT TRUNCATED AT 250 WORDS) | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 7970942 |
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