The fractionation of human plasma proteins. II. The purification of human complement proteins C3, C3u, and C5 by application of affinity chromatography. Protein expression and purification [Protein Expr Purif] Journal article

Title	The fractionation of human plasma proteins. II. The purification of human complement proteins C3, C3u, and C5 by application of affinity chromatography.
Author(s)	DiScipio RG, Sweeney SP
Institution	Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
Source	Protein Expr Purif 1994 Apr; 5(2):170-7.
MeSH	Blood Proteins Chemical Fractionation Chromatography, Affinity Chromatography, Ion Exchange Complement C3 Complement C5 Complement Factor B Complement Factor H Complement System Proteins Dextran Sulfate Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G
Abstract	We have devised a purification scheme for human complement proteins C3, C3u, and C5. Affinity chromatography is employed to isolate each of these three proteins in a highly purified form. Typical yields were 910, 30, and 53 mg for C3, C3u, and C5, respectively, from 3 liters of human plasma. These yields reflect 31 and 32% recoveries for C3 and C5, respectively. The major advantages of this protocol over other published procedures are the application of affinity chromatography to yield high quality purified complement components, and the fact that the purification of C3, C3u, and C5 is part of a broader scheme designed for the isolation of over 20 plasma proteins from a single batch of plasma.
Language	eng
Pub Type(s)	Journal Article Research Support, U.S. Gov't, P.H.S.
PubMed ID	8054851

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