| Title | The Penicillium chrysogenum and Aspergillus nidulans wetA developmental regulatory genes are functionally equivalent. | | Author(s) | Prade RA, Timberlake WE | | Institution | Dept. of Genetics, University of Georgia, Athens 30602-7223. | | Source | Mol Gen Genet 1994 Sep 1; 244(5):539-47. | | MeSH | Amino Acid Sequence
Aspergillus nidulans
Base Sequence
Conserved Sequence
DNA Mutational Analysis
DNA Primers
DNA, Fungal
Fungal Proteins
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Regulator
Molecular Sequence Data
Morphogenesis
Penicillium chrysogenum
Phylogeny
Promoter Regions (Genetics)
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Spores, Fungal
| | Abstract | Aspergillus nidulans and Penicillium chrysogenum are related fungi that reproduce asexually by forming multicellular conidiophores and uninucleate conidia. In A. nidulans, spore maturation is controlled by the wetA (AwetA) regulatory gene. We cloned a homologous gene (PwetA) from P. chrysogenum to determine if spore maturation is regulated by a similar mechanism in this species. The PwetA and AwetA genes are similar in structure and functional organization. The inferred polypeptides share 77% overall amino acid sequence similarity, with several regions having > 85% similarity. The genes also had significant, local sequence similarities in their 5' flanking regions, including conserved binding sites for the product of the regulatory gene abaA. PwetA fully complemented an A. nidulans wetA deletion mutation, demonstrating that PwetA and its 5' regulatory sequences function normally in A. nidulans. These results indicate that the mechanisms controlling sporulation in A. nidulans and P. chrysogenum are evolutionarily conserved. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 8078481 |
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