| Title | Inhibition of in vitro tumor cell invasion by ginsenoside Rg3. | | Author(s) | Shinkai K, Akedo H, Mukai M, Imamura F, Isoai A, Kobayashi M, Kitagawa I | | Institution | Department of Tumor Biochemistry, The Center for Adult Diseases Osaka, Japan. | | Source | Jpn J Cancer Res 1996 Apr; 87(4):357-62. | | MeSH | Adenocarcinoma
Animals
Antineoplastic Agents
Carbohydrate Sequence
Carcinoma, Small Cell
Ginsenosides
Humans
Liver Neoplasms, Experimental
Lung Neoplasms
Lysophospholipids
Melanoma, Experimental
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Proteins
Pancreatic Neoplasms
Phosphorylation
Rats
Rats, Inbred Strains
Research Support, Non-U.S. Gov't
Saponins
Tyrosine
| | Abstract | The effect of plant glycosides on tumor cell invasion was examined. Among the glycosides tested, ginsenoside Rg3 was found to be a potent inhibitor of invasion by rat ascites hepatoma cells (MM1), B16FE7 melanoma cells, human small cell lung carcinoma (OC10), and human pancreatic adenocarcinoma (PSN-1) cells, when examined in a cell monolayer invasion model. Structurally analogous ginsenosides, Rb2, 20(R)-ginsenoside Rg2 and 20(S)-ginsenoside Rg3 (a stereoisomer of Rg3), showed little inhibitory activity. Neither Rh1, Rh2, 20(R)-ginsenosides Rh1, Rb1, Rc nor Re had any effect. The effective ginsenoside, Rg3, tended to inhibit experimental pulmonary metastasis by highly metastatic mouse melanoma B16FE7 cells as well. Taking account of our previous finding that 1-oleoyl-lysophosphatidic add (LPA) induced invasion by MM1 cells in the monolayer invasion model, the effect of Rg3 on molecular events associated with the invasion induced by LPA was analyzed in order to understand the mechanism of the inhibition. Rg3, which suppressed the invasion induced by LPA, dose-dependently inhibited the LPA-triggered rise of intracellular Ca2+. Protein tyrosine phosphorylation triggered by LPA was not inhibited by Rg3. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 8641966 |
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