| Title | [Interference of factor VIII excess on the detection of lupus anticoagulants by the activated partial thromboplastin time] | | Author(s) | Guermazi S, Gorgi Y, Ayed K, Dellagi K | | Institution | Laboratoire d'Hématologie, Institut Pasteur de Tunis. | | Source | Pathol Biol (Paris) 1997 Jan; 45(1):28-33. | | MeSH | Adolescent
Adult
Antibodies, Antiphospholipid
Child
Child, Preschool
Drug Interactions
Factor VIII
Female
Humans
Lupus Coagulation Inhibitor
Lupus Erythematosus, Systemic
Male
Middle Aged
Partial Thromboplastin Time
von Willebrand Factor
| | Abstract | Antiphospholipids antibodies (AAP) were investigated in plasma of 62 patients with systemic lupus erythematosus, and studied for the possible interference of the excess in factor VIIIc-Von Willebrand factor (VWF-VIIIc) complex in the detection of lupus anticoagulants (LA) by the activated partial thromboplastin time (APTT). We used four commercial reagents and noted that each one exhibited a different level of sensitivity of LA; the most sensitive one allowed the detection of 16 LA positive plasmas while the less sensitive reagent detected only 6 positives. The Elisa test for IgG and IgM AAP detection was positive in 25 out of 62 plasmas (40%). Comparison of the Von Willebrand factor antigen level and the APTT values showed a significant negative corrélation with 2 reagents (r = 0.369, p < 0.01 and r = 0.272, p < 0.05 respectively) that were also the less sensitive to LA. The interference of an excess in VWF-VIIIc complex was further studied by the addition of purified factor VIII in 3 LA positive plasmas. Our results suggests that an excess of factor VIIIc could lead to false negative LA, using certain APTT reagents. We conclude that a more accurate LA detection require: sensitive reagents, a pool of normal plasmas selected with normal factor VIII level, as well as repeated testing of blood sampling withdrawn away of an inflammatory process to avoid false negative LA detection. | | Language | fre | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 9097843 |
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