Cardiac troponin I, cardiac troponin T, and creatine kinase MB in dialysis patients without ischemic heart disease: evidence of cardiac troponin T expression in skeletal muscle. Clinical chemistry. [Clin Chem] Journal article | | Title | Cardiac troponin I, cardiac troponin T, and creatine kinase MB in dialysis patients without ischemic heart disease: evidence of cardiac troponin T expression in skeletal muscle. | | Author(s) | McLaurin MD, Apple FS, Voss EM, Herzog CA, Sharkey SW | | Institution | Department of Medicine, Hennepin County Medical Center, Minneapolis, MN 55415, USA. | | Source | Clin Chem 1997 Jun; 43(6 Pt 1):976-82. | | MeSH | Adult
Aged
Aged, 80 and over
Biological Markers
Biopsy
Blood Urea Nitrogen
Comparative Study
Creatine Kinase
Enzyme-Linked Immunosorbent Assay
Female
Humans
Isoenzymes
Kidney Failure, Chronic
Male
Middle Aged
Muscle, Skeletal
Myocardial Ischemia
Myocardium
Renal Dialysis
Research Support, Non-U.S. Gov't
Troponin
Troponin I
Troponin T
| | Abstract | Serum cardiac troponin T (cTnT) concentrations are frequently increased in chronic dialysis patients as measured by the first-generation ELISA immunoassay, as is creatine kinase (CK) MB mass in the absence of acute ischemic heart disease. We designed this study to compare four serum markers of myocardial injury [CK-MB mass, first-generation ELISA cTnT, second-generation Enzymun cTnT, and cardiac troponin I (cTnI)] in dialysis patients without acute ischemic heart disease. We also evaluated skeletal muscle from dialysis patients as a potential source of serum cTnT. No patients in the clinical evaluation group (n = 24) studied by history and by physical examination, electrocardiography, and two-dimensional echocardiography had evidence of ischemic heart disease. Biochemical markers were measured in serial predialysis blood samples with specific monoclonal antibody-based immunoassays. For several patients at least one sample measured above the upper reference limit: CK-MB, 7 of 24 (30%); ELISA cTnT, 17 of 24 (71%); Enzymun cTnT, 3 of 18 (17%); and cTnI, 1 of 24 (4%). In a separate group of dialysis patients (n = 5), expression of cTnT, but not cTnI, was demonstrated by Western blot analysis in 4 of 5 skeletal muscle biopsies. Chronic dialysis patients without acute ischemic heart disease frequently had increased serum CK-MB and cTnT. The specificity of the second-generation cTnT (Enzymun) assay was improved over that of the first-generation (ELISA) assay; cTnI was the most specific of the currently available biochemical markers. cTnT, but not cTnI, was expressed in the skeletal muscle of dialysis patients. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 9191549 |
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