| Title | Transforming growth factor-beta receptor types I and II are expressed in renal tubules and are increased after chronic unilateral ureteral obstruction. | | Author(s) | Sutaria PM, Ohebshalom M, McCaffrey TA, Vaughan ED, Felsen D | | Institution | James Buchanan Brady, Department of Urology, Cornell University Medical College, New York, NY 10021, USA. | | Source | Life Sci 1998; 62(21):1965-72. | | MeSH | Actins
Animals
Gene Expression
Immunoenzyme Techniques
Kidney Cortex
Kidney Medulla
Kidney Tubules
Polymerase Chain Reaction
RNA, Messenger
Rats
Rats, Sprague-Dawley
Receptors, Transforming Growth Factor beta
Ureteral Obstruction
| | Abstract | Transforming growth factor-beta (TGF-beta) is a profibrotic cytokine which has been implicated in the renal fibrosis which follows unilateral ureteral obstruction (UUO) in the rat. TGF-beta receptor type I (TGF-RI) and TGF-beta receptor type II (TGF-RII) are part of the complex which mediates the response to TGF-beta. We sought to determine if TGF-RI and TGF-RII are found in the kidney, and if their expression is changed as a result of UUO. Polymerase chain reaction (PCR) was used to determine expression of mRNA for TGF-RI and TGF-RII in the kidney. Immunoperoxidase was used to localize and quantify the expression of these receptors at 3, 7, 14, 21 and 28 days after UUO, and in sham-operated animals. Expression of mRNA for TGF-RI and TGF-RII was demonstrated in sham operated, obstructed and contralateral unobstructed kidneys using PCR. Using immunoperoxidase, a uniform distribution of TGF-RI and TGF-RII was found in cortical tubules of sham operated kidneys, whereas medullary tubules showed a patchy TGF-RI distribution and no TGF-RII staining. After UUO, an increased tubular expression of TGF-RI and TGF-RII was noted in both obstructed and contralateral kidneys compared to sham operated kidneys. No staining for either TGF-RI or TGF-RII was noted in glomeruli, vasculature or interstitial cells. TGF-beta receptors I and II were found exclusively in renal tubules and were shown to increase in both the obstructed and contralateral kidneys relative to sham operated animals. Upregulation of TGF-beta receptors in both kidneys suggests that TGF-beta may contribute to the fibrotic response in the obstructed kidney and the hypertrophic response of the contralateral kidney. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 9619846 |
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