Dual regulatory effects of interferon-alpha, -beta, and -gamma on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1alpha, or tumor necrosis factor-alpha. Journal of dental research. [J Dent Res] Journal article | | Title | Dual regulatory effects of interferon-alpha, -beta, and -gamma on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1alpha, or tumor necrosis factor-alpha. | | Author(s) | Sakuta T, Tokuda M, Tamura M, Jimi E, Ikebe T, Koba T, Nagaoka S, Takada H | | Institution | Department of Microbiology and Immunology, Kagoshima University Dental School, Japan. | | Source | J Dent Res 1998 Aug; 77(8):1597-605. | | MeSH | Blotting, Northern
Cells, Cultured
Child
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Gene Expression Regulation
Gingiva
Humans
Interferon Type II
Interferon-alpha
Interferon-beta
Interferons
Interleukin-1
Interleukin-8
Lipopolysaccharides
Male
NF-kappa B
Prevotella intermedia
Protein Synthesis Inhibitors
RNA, Messenger
Recombinant Proteins
Research Support, Non-U.S. Gov't
Tosylphenylalanyl Chloromethyl Ketone
Tumor Necrosis Factor-alpha
Up-Regulation
| | Abstract | In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-beta or -gamma). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by human gingival fibroblasts. Priming with IFN-alpha (alpha), -beta, or -gamma upregulated the IL-8 mRNA expression in response to P. intermedia LPS, whereas co-stimulation with these IFNs reduced the amount of mRNA expressed by the cells. The regulation of IL-8 mRNA expression induced by recombinant human tumor necrosis factor-alpha (rHuTNF-alpha) or rHuIL-1alpha was similar to that induced by LPS. The IL-8 mRNA expression in response to P. intermedia LPS was enhanced by IFN-gamma independently of de novo protein synthesis, and was regulated, at least in part, at the transcriptional level. The IL-8 mRNA accumulation in response to P. intermedia LPS was inhibited by tosylphenyl-alanyl chloromethyl-ketone, an inhibitor of NF-kappaB activation, although the NF-kappaB activation itself was not altered by IFN-gamma. These findings suggest that IFNs might be capable of both enhancing and inhibiting inflammatory responses in periodontal tissues through the dual regulation of IL-8 production by gingival fibroblasts in response to bacterial components and cytokines. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 9719033 |
|
|
| |
Advertise on this site.
| | |
|
