Non-alcoholic fatty liver disease (NAFLD), often associated with obesity, has become a serious public health matter. NAFLD is characterized by an excessive lipid accumulation in hepatocytes, mainly stored as triglycerides. The marine microalga Phaeodactylum tricornutum is well known for its richness of bioactive compounds, particularly lipids. Therefore, different natural lipid extracts from P. tricornutum are deciphered to jugulate or prevent obesity leading to NAFLD. In this study, the main focus was on the effects of purified neutral and polar lipid extracts from P. tricornutum in a cellular model of NAFLD. Human HepG2 cells were used and exposed for 24 h to 250 μM palmitate to induce NAFLD with or without microalgal lipid extracts. Data showed that neutral lipid extract presented lower viability and cytotoxic activities on HepG2 at 75 µg/mL. The impact on apoptosis was around 5% and below the threshold. Nevertheless, the use of neutral lipid at 50 µg/mL induced a decrease in the number and size of lipid droplets, and so, preventing NAFLD. On the contrary, the polar lipid extract had no effect on the accumulation of triglycerides in HepG2 cells. To conclude, neutral lipid extract seemed to be a good candidate to prevent NAFLD.

The present article represents findings of quality of life index dynamics for 100 patients with gastroesophageal reflux disease under pressure of proton pump inhibitor therapy. The degree of clinical evidence was measured by Likert scale, quality of life appraisal was made on the bases of SF-36, GSRS checklists, psychoemotional component of patients' health was analyzed with the help of the V.M. Bekhterev Institute personal enquirer. Treatment with Rabeprazole helps efficiently jugulate reflux disease symptoms and accurately improve quality of life index.

Frequent selection of mutants resistant to extended and broad spectrum cephalosporins in Enterobacter cloacae is observed in hospital. As we noticed an unusual number of isolates of these strains and to answer the question arose whether these Enterobacter had a common source, we retrospectivally studied 56 strains collected in 11 wards of the hospital. Using PFGE with Spe 1 restriction analysis we identified a prevalent clone (11 patients) dispatched in 8 wards. We also obviously proved cross-contamination patients to patients with other clones. PFGE allow us to point out that clonal Enterobacter cloacae has taken place in our hospital, even if there is no real outbreak. A reinforcement of basic hygienic measures and a control of antibiotics prescription seemed very important to jugulate the sudden increase of multiresistant Enterobacter cloacae prevalence.