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(Molecular cellular proteomics[TA])
3,136 results
  • Robust Label-Free, Quantitative Profiling of Circulating Plasma Microparticle Associated Proteins. [Journal Article]
  • MCMol Cell Proteomics 2016 Oct 12
  • Braga-Lagache S, Buchs N, … Heller M
  • CONCLUSIONS: Using aliquots of 250 μL platelet-free plasma from one individual donor, we achieved excellent reproducibility with an inter-assay coefficient of variation of 2.7 ± 1.7% (mean ± 1 standard deviation) on individual peptide intensities across 27 acquisitions performed over a period of 3.5 months. We show that the microparticle proteome between twelve healthy volunteers were remarkably similar, and that it is clearly distinguishable from whole cell and platelet lysates. We propose the use of the proteome profile shown in this work as a quality criterion for microparticle purity in proteomics studies. Furthermore, one freeze thaw cycle damaged the microparticle integrity, articulated by a loss of cytoplasm proteins, encompassing a specific set of proteins involved in regulating dynamic structures of the cytoskeleton, and thrombin activation leading to MP clotting. On the other hand, plasma membrane protein composition was unaffected. Finally, we show that multiplexed data-independent acquisition can be used for relative quantification of target proteins using Skyline software. Mass spectrometry data are available via ProteomeXchange with (identifier PXD003935) and
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