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Ross Fiziol Zh Im I M Sechenova [journal]
- Concerning a clue on the enigma of "amyloid made" versus "amyloid regulated" channels in cell membranes: a comment on M. A. Mukhamedyarov et al. NBP. 43: 479-484. 2013. [Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):1002-4.
- [Studying of the role of tumor necrosis factor-alpha in regulation of erythropoiesis in erythroblastic islands cultures]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):993-1001.
An elevated level of tumor necrosis factor-alpha in a cultural system of bone marrow erythroblastic islands was found. It was associated with the suppressed formation of erythroblastic islands de repeto in 24-hr cultures. In 48-hr cultures, an increased level of erythropoietin and intensive formation of erythroblastic islands de repeto and de novo was found. In 72-hr cultures, further elevation of TNF-alpha was accompanied by a decrease of erythroblastic islands in culture.
- [Amyloid-like oligomers of presynaptic protein BASP1]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):984-92.
Brain protein BASP1 forms oligomers that resemble amyloid protein oligomers in some respects, including interaction with conformation-specific antibodies against amyloid oligomers. Aggregation-prone N-terminal myristoylated peptide myr-BASP1 (1-13) forms fibrillar aggregates of amyloid-like structure under physiological conditions in vitro, which is also the evidence of BASP1 similarity to amyloid proteins. Protein cross-linking by glutaraldehyde in situ demonstrated that BASP1 exists on the presynaptic membrane as lipid raft-associated oligomers. In addition, BASP1 is non-toxic to PC12 cells, when applied either as a monomer or oligomer. We conclude that BASP1 oligomer is a non-pathological physiologically relevant functional form of this protein.
- [Inhibition of Bcl-2 stimulates neuronal stem proliferation in organotypic cultures of mice hippocampus]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):976-83.
In the current study, we investigated the participation of Bcl-2 in both processes of hippocampus neuronal stem cells (NSC) proliferation and differentiation. Present experiments are performed on organotypic cultures of mice hippocampus. A selective inhibitor Bcl-2 HA14-1 (10 μM) is supplied in incubating medium and the concentration is maintained at a constant level. Our data demonstrate that per cent of surviving cells is significantly higher in the group with the supplement HA14-1 then in the control group. In additional, expressions both phospho-histone H3 and Oct3/4 significantly increase in the group with supplement HA14-1. The facts suggest about activation of NSCs proliferation. After 6 weeks incubation, formation of embryoid bodies is observed in the group with HA14-1, that also suggest about NSCs proliferation, but not their differentiation. Also we estimate the level of NSCs differentiation. Our data have shown that the level of CRMP-2 (a protein which participates in axon growth during NSCs differentiation) decreases in the group with HA14-1. We also estimate level of ERK1/2 kinase activity of the MAPK signaling pathway, which immediately regulates neuronal differentiation. Decreasing of both activities ERK1/2 and CRMP-2 indicates diminution of neuronal differentiation in the experimental group. Thus, we demonstrate that inhibition of Bcl-2 increasingly stimulates NSCs proliferation, so that, it suggests that Bcl-2 controls NSCs differentiation to neurons.
- [Attraktive and aversive for intact individuals volatile excretion of male mice induced a thymus dependent antigen]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):968-75.
Immunization of male mice of the CBA and C57BL/6J strains with sheep red blood cells (SRBC) induce excretion of urinary volatile components (VC), modifying the behavioral reactions of intact males CBA strain. In early times after injection of 1 x 10(8) SRBC per mouse results in secretion of attractive VC, whereas injection of 1 x 10(9) cells per mice induces secretion of aversive VC. Regular for normal and the observed also in the early stages of attraction of intact males CBA to VC immunized syngeneic males CBA (relatively VC immunized with allogeneic C57BL/6) was lost from 3 to 14th day after the introduction of SRBC in the dose of 1 x 10(8) cells/mice and with the 9th on the 14th day after the introduction of SRBC in the dose of 1 x 10(9). The biological significance of the antigen induced chemosignaling in animals is discussed.
- [Potentiative effects of noradrenaline on the neurogenic vasoreactivity diminished by cooling]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):960-7.
The effect of 0.03--1.0 μM noradrenaline on the response to electrical field stimulation of the juvenile rat tail artery segment at 36 degrees C and after cooling to 25 degrees C was studied. At 25 degrees C, the neurogenic vasoconstriction was inhibited, but low dose noradrenaline potentiate the constriction. This potentiation was greater at 25 degrees C than at 36 degrees C, following spontaneous decline in the constriction counteracted by noradrenaline. At low temperature, the potentiative effects of noradrenaline were greater at high frequency of electrical field stimulation. The phenomenon of increase in the noradrenaline-evoked potentiation of neurogenic vasoconstriction inhibited by cold may be of importance for thermoregulation. It could provide restoration of diminished effectiveness of the neurogenic contractile signal thus leading to low heat emission at low temperature.
- [Urine proteome study for the evaluation of cardiovascular system state after spaceflight in human]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):945-59.
In order to find markers to assess the functional state of the cardiovascular system before and after spaceflight (first and seventh day after landing), we analyzed the urine proteome in ten cosmonauts aged of 35 to 51 years who have completed 169 to 199-day spaceflight onboard the ISS. A special sample preparation was performed, followed by liquid chromatography-mass spectrometry. Liquid chromatography-mass spectrometry of the minor proteins was performed on a nano-HPLC Agilent 1100 system (Agilent Technologies Inc., USA) in combination with a LTQ-FT Ultra mass spectrometer (Thermo, Germany). A total of 238 proteins was identified. According to the TIGER database, a tissue origin was established for 129 proteins. We identified 20 proteins related to the cardiovascular system. It was found that changes in cosmonauts' urine proteome comprehensively reflect the adaptive responses of cardiovascular, renal and neuroendocrine systems to long-term microgravity conditions.
- [Effect of the sharply strengthened motor activity on heart pumping ability of rats and mechanisms of its regulation]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):937-44.
The indicators of heart pumping ability of rats at a muscular loading of the maximum power and also in the conditions of transition from sharply strengthened motor activity regime on a strengthened motor activity regime at adrenergic influence stimulation and blockade were investigated. At rats of 100-daily age at the strengthened motor activity heart rate is less, and blood stroke volume is more, than in the rats, subject to muscular loading of the maximum power. The adrenergic influence on the heart's pumping ability of sharply strengthened motor activity rats is much more, than of unlimited motor activity rats. At the α1-adrenoreceptors blockade at 100-daily rats the decreasing in intensity of muscular loading causes increased in adrenergic influence on heart pumping ability.
- [The contrast sensitivity in schizophrenia with different durations disease]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):928-36.
We recorded contrast sensitivity in normal and schizophrenic patients. We presented Gabor gratings with sinusoidal distribution of brightness, with the spatial frequency: 0.4 cycles/degree, 3.6 and 17.9 cycles/degree. Taken together patients with schizophrenia compared to the healthy subjects showed a decline of contrast sensitivity at low and medium spatial frequencies. We measured the influence of duration of illness and type of treatment on contrast sensitivity. Patients with schizophrenia less than 10 years, showed a decrease of contrast sensitivity only at low spatial frequencies, while patients suffered with schizophrenia for more than 10 years--at low, medium and high spatial frequencies. Persons with schizophrenia less than 10 years receiving treatment with atypical antipsychotics, show a decrease in contrast sensitivity at low spatial frequencies, while treated with typical antipsychotics--in the low and high spatial frequencies.
- [Non-invasive transcutaneous spinal cord stimulation facilitates locomotor activity in decerebrated and spinal cats]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2013 Aug; 99(8):917-27.
It is known that spinal neuronal networks activated by epidural electrical stimulation (EES) can produce the stepping EMG pattern and control the locomotor behavior. At present study we showed that non-invasive transcutaneous electrical spinal cord stimulation (tESCS) applied to the lumbar-sacral enlargement can facilitate the locomotor activity in decerebrated and spinal animals. The comparison of the motor responses evoked by EES vs tESCS showed that both methods produce the locomotor patterns with close properties and similar reflex mechanisms. The data obtained suggest that tESCS is an efficient approach for investigation of the locomotor control in acute and chronic experiments as well as facilitates of the locomotor abilities after spinal cord injury. Taking to account the non-invasivity and easement of tESCS, this approach could be further implemented in clinical practice for rehabilitation of the patient with spinal cord injury.