Ross Fiziol Zh Im I M Sechenova [journal]
- [THE INVESTIGATION OF VASOPRESSIN SECRETION IN NORMAL CONDITION AND DURING SEIZURE ONSET IN KRUSHINSKY-MOLODKINA RATS]. [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
- Ross Fiziol Zh Im I M Sechenova 2015 Dec; 101(12):1347-54.
The aim of our study was to analyze vasopressin secretion rate from hypothalamic neurons in Krushinsky-Molodkina (KM) rats prone to audiogenic epilepsy in control and during audiogenic seizures. We evaluated vasopressin content in blood serum and neurophysin II amount in the neurohypophysis in KM rats as well as in Wistar rats. Obtained data demonstrated decreasing of vasopressin in the blood and at the same time increasing of neurophysin II content in the neurohy- pophysis of KM rats that revealed an inhibition of vasopressin release into blood circuit. The analysis of vasopressin content in blood on the different stages of audiogenic seizure showed significant increasing of vasopressin at clonustonus. Thus, we obtained first demonstration that in KM rats prone to audiogenic seizures vasopressin secretion rate is decreased in comparison to Wistar rats. Significantly upregulated vasopressin in blood at cloniconic stages reveals a participation of vasopressinergic neurosecretory system in the expression of audiogenic seizures.
- [SKIERS URINARY CATECHOLAMINES EXCRETION AT REST AND BY COMPETITIVE LOADS LENGTH VARIETY]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1324-30.
While night sleeps urinary noradrenaline excretion of skilled skiers less than their untrained peers, but the difference in excretion of adrenaline is not revealed. By increasing the distance and time to overcome it during skiing catecholamine excretion is increased both - totally and per minute. Most urinary catecholamines detected at a distance of 50 km in low sliding: increased excretion of adrenaline - 84 times, and noradrenaline -95 times. These results shows that high qualificated skier's functional reserve of the sympathoadrenal system, is mobilize at long competitions for ten times higher than in rest.
- [THE INFLUENCE OF SEROTONIN TRANSPORTER AND MONOAMINE OXIDASE A GENES POLYMORPHISM ON PSYCHO-EMOTION AND KARYOLOGICAL STABILITY OF ATHLETES]. [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1309-23.
The influence of polymorphism of the serotonin transporter and monoamine oxidase A genes, associated with man's aggressiveness on the psycho-emotional state and karyological status of single combat athletes. It was revealed that the carriers of less active ("short"), monoamine oxidase A gene variant have a high motivation to succeed and less rigidity and frustrated, compared to the carriers of more active ("long") version of the gene. Heterozygote carriers of less active ("short") variant of the serotonin transporter gene 5-HTTL had more physical aggression, guilt and were less frustrated compared with carriers of two long alleles. It has been revealed the association of studied genes with the karyological status of athletes. So fighters who are carriers of the short and long alleles of the serotonin transporter gene had more cells with nuclear abnormalities in the buccal epithelium than single combat athletes which both alleles were long.
- [SIGNALING PATHWAYS REGULATING PROTEIN SYNTHESIS IN RAT SOLEUS MUSCLE DURING EARLY STAGES OF HINDLIMB UNLOADING]. [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1299-308.
The impact of mechanical unloading (hindlimb suspension for 1-, 3- and 7 days) on the protein synthesis intensity and as well as intracellular signaling pathways controlling mRNA translation was investigated. The content of the key signaling molecules of the various anabolic signaling pathways was determined by Western-blotting. The rate of protein synthesis was assessed using in vivo SUnSET technique. Hindlimb suspension (HS) within 24 hours resulted in a significant (p < 0.05) decrease in p-4E-BP 1 content and increase in p-p70s6k content in soleus muscle. Follo- wing three days of HS the content of p-AKT and p-90RSK1 was decreased (p <0.05). After 7 days of HS phosphorylation level of AKT and GSK-3beta was significantly reduced (p < 0.05) compared to the control group. We also observed a significant decrease in the amount of 28S rRNA in soleus following 3 and 7 days of HS. The rate of protein synthesis after 3 and 7 days of HS was decreased (p < 0.05) in comparison with the basal level. Our data suggest that the decrease in the protein synthesis rate in rat soleus during early stages of simulated microgravity could be associated with the impaired ribosome biogenesis as well as reduced activity of the mTORC1-independent signaling pathways.
- [THE INDICATORS OF GROWTH AND MYOGENESIS IN THE GLYCOLYTIC AND OXIDATIVE MUSCLES OF THE RAT AFTER INDIRECT ELECTROSTIMULATION]. [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1289-98.
A comparative analysis of the signaling pathways activity and gene expression in the red (RG) and white (WG) parts of the gastrocnemius muscle of rat after a series of short (1 s) tetanic contractions induced by motor nerve stimulation at a frequency of 100 Hz and with an amplitude that provides activation of all motor units of the muscle. WG compared to RG demonstrated a marked increase in the phosphorylation level of ERK1/2, although the increase in the phosphorylation of AMPK was not different in two muscles 2 h after the stimulation. Along with that, content of MyoD and myogenin mRNA in WG increased much higher than in RG, whereas the effect of stimulation on IGF-1, MaFbx and MuRF genes expression was weak and comparable in WG and RG. There was an increase of myostatin mRNA in RG. Thus, glycolytic muscle fibers of WG exhibit more pronounced regulatory shifts of hypertrophic character than oxidative muscle fibers of RG.
- [ELECTRIC STIMULATION OF VAGUS NERVE MODULATES A PROPAGATION OF OXYGEN EPILEPSY IN RABBITS]. [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1279-88.
The activation of autonomic afferents (achieved through the vagus nerve (VN) electrical stimulation) on CNS O2 toxicity and cardiovascular function was investigated. In conscious rabbits at 5 ATA 02, prodromal signs of CNS O2 toxicity and convulsion latency were determined with and without vagus nerve (VN) stimulation. EEG, ECG and respiration were also recorded. In rabbits at 5 ATA, sympathetic overdrive and specific patterns on the EEG (synchronization of slow-waves), ECG (tachycardia) and respiration (respiratory minute volume increase) preceded motor convulsions. Vagus nerve stimulation increased parasympathetic component of autonomic drive and significantly delayed prodromal signs of oxygen toxicity and convulsion latency. Autonomic afferent input to the brain is a novel target for preventing CNS toxicity in HBO2.
- [CROSS-TALK BETWEEN 5-HT1A AND 5-HT7 RECEPTORS: ROLE IN THE AUTOREGULATION OF THE BRAIN SEROTONIN SYSTEM AND IN MECHANISM OF ANTIDEPRESSANTS ACTION]. [English Abstract, Journal Article, Review]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1270-8.
Recent studies considerably extended our knowledge of the mechanisms and physiological role of the interaction between different receptors in the brain. Current review summarizes data on the formation of receptor complexes and the role of such complexes in the autoregulation of the brain serotonin system, behavioral abnormalities and mechanism of antidepressants action. Particular attention is paid to 5-HT1A and 5-HT7 receptor heterodimers. The results described in the present review indicate that: i) dimerization and formation of mobile receptor complexes is a common feature for the members of G-protein coupled receptor superfamily; ii) 5-HT7 receptor appears to be a modulator for 5-HT1A receptor - the key autoregulator of the brain serotonin system; iii) 5-HT1A/5-HT7 receptor complexes formation is one of the mechanisms for inactivation and desensitization of the 5-HTIA receptors in the brain; iv) differences in the 5-HT7 receptor and 5-HTIA/5-HT7 heterodimers density define different sensitivity of pre- and postsynaptic 5-HTlA receptors to chronic treatment with selective serotonin reuptake inhibitors.
- [THE EFFECT OF NEUROPEPTIDE Y ON SPIKE ACTIVITY OF NEURONS IN THE SUPRACHIASMATIC NUCLEUS OF RAT IN VITRO]. [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1257-69.
In the experiments on sagittal hypothalamic slices of male Wistar rats, the effect of 10 nM neuropeptide Y on the electrical activity of the suprachiasmatic nucleus neurons and spike information coding parameters were studied. Applications of neuropeptide Y induced a reduction of action potential frequency in 35 of 81 tested neurons, in 8 cells an increase in the parameter was observed; the remaining 38 neurons did not change their spike activity level. The decrease in spike frequency generation by neurons of the suprachiasmatic nucleus accompanied by an increase in both entropy of interspike interval distribution and mutual information between adjacent interspike intervals. These suggest an increased irregularity of the later as well as an increased patterning of spike information induced by neuropeptide Y. The obtained data indicate the ability of neuropeptide Y to modulate a level of activity and influence a spike code in a relatively numerous population of neurons in circadian oscillator of the suprachiasmatic nucleus.
- [THE STUDY OF THE TEMPORAL PROPERTIES OF THE AUDITORY SENSORY TRACE FOR A SHORT DURATION STIMULI USING THE MISMATCH NEGATIVITY]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1250-6.
The present article accesses the dependence of mismatch negativity of the human auditory event related potentials on the length of interstimulus interval. We employed passive oddball paradigm as a presentation method and simple tones were used as stimuli. This study was based on the assumption, that storage time of the memory trace, which is used for generation of mismatch negativity, is dependent on the length of the stimulus itself. As a result, the mismatch negativity was registered for short durations 11 ms stimuli. In such stimuli differences of amplitude of mismatch negativity depending on the length of interstimulus interval were also shown. Yet no such differences were found for stimuli of 20 ms and 30 ms. This result allows us to assume that storage time of memory trace of stimulus is directly proportional to the length of the stimulus itself when short interstimulus intervals are used. However, the increase of stimulus length over certain threshold does not show any changes in the properties of mismatch negativity.
- [CHANGES IN THE QUALITATIVE AND QUANTITATIVE COMPOSITION OF GUT MICROBIOTA IN RATS DURING EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS]. [English Abstract, Journal Article]
- Ross Fiziol Zh Im I M Sechenova 2015 Nov; 101(11):1235-49.
In this study, on the model of multiple sclerosis - experimental allergic encephalomyelitis (EAE), the dynamics of changes in the qualitative and quantitative composition of the intestinal microbiota in rats with symptoms of the disease and asymptomatic course were compared. It was found that the composition of the intestinal microbiota in rats with the clinical symptoms of EAE is shifted towards gram-negative opportunistic microorganisms of the genus Citrobacter, Prote- us, Klebsiella and enteropathogenic Escherichia coli. It has been shown that rats without clinical signs of EAE have higher levels of Faecalibacteriumprausnitzii. The significance of the complex changes in the composition of the intestinal microbiota, indicating long-lasting dysbiosis in rats during the development of EAE is discussing.