- Single-Dose Adductor Canal Block With Local Infiltrative Analgesia Compared With Local Infiltrate Analgesia After Total Knee Arthroplasty: A Randomized, Double-Blind, Placebo-Controlled Trial. [Journal Article]
- RAReg Anesth Pain Med 2016 Nov/Dec; 41(6):678-684
- CONCLUSIONS: Adductor canal blockade with bupivacaine 0.25% with epinephrine 1:300,000 effectively reduces pain and opioid requirement in the postoperative period after TKA. Adductor canal blockade is an effective pain management adjunct for patients undergoing TKA.
- Correction of impaired calmodulin binding to RyR2 as a novel therapy of lethal arrhythmia in the pressure-overloaded heart failure. [Journal Article]
- HRHeart Rhythm 2016 Oct 19
- CONCLUSIONS: Several disorders in the RyR channel function characteristic of pressure-overloaded cells (increased spontaneous Ca(2+) leakage, delayed after-depolarization, triggered activity, Ca(2+) spark frequency, sCaT) are caused by deteriorated CaM binding to the RyR2. These disorders could be rectified by restoring normal CaM binding to the RyR2.
- Sodium Nitroprusside Enhanced Cardiopulmonary Resuscitation Improves Short Term Survival in a Porcine Model of Ischemic Refractory Ventricular Fibrillation. [Journal Article]
- RResuscitation 2016 Oct 19
- CONCLUSIONS: SNPeCPR significantly improved ROSC and 4-hour survival compared with standard ACLS CPR in a porcine model of prolonged ischemic, refractory VF cardiac arrest.
- Differential expression of putative adhesin genes of Actinobacillus suis grown in in vivo-like conditions. [Journal Article]
- VMVet Microbiol 2016 Nov 15; 195:60-69
- Actinobacillus suis is an opportunistic pathogen that resides in the tonsils of the soft palate of swine. Unknown stimuli can cause this organism to invade the host, resulting in septicaemia and sequ...
Actinobacillus suis is an opportunistic pathogen that resides in the tonsils of the soft palate of swine. Unknown stimuli can cause this organism to invade the host, resulting in septicaemia and sequelae including death. To better understand its pathogenesis, the expression of several adhesin genes was evaluated by semi-quantitative real-time PCR in A. suis grown in conditions that mimic the host environment, including different nutrient and oxygen levels, exponential and stationary phases of growth, and in the presence of the stress hormone epinephrine. Fifty micromolar epinephrine did not affect the growth rate or expression of A. suis adhesin genes, but there was a significant growth phase effect for many genes. Most adhesin genes were also differentially expressed during anoxic static growth or aerobic growth, and in this study, all genes were differentially expressed in either exponential or stationary phase. Based on the time*treatment interactions observed in the anoxic study, a model of persistence of A. suis in the host environment in biofilm and planktonic states is proposed. Biofilm dynamics were further studied using wild type and isogenic mutants of the type IVb pilin (Δ flp1), the OmpA outer membrane protein (ΔompA), and the fibronectin-binding (ΔcomE1) genes. Disruption of these adhesin genes affected the early stages of biofilm formation, but in most cases, biofilm formation of the mutant strains was similar to that of the wild type by 24h of incubation. We postulate that other adhesins may have overlapping functions that can compensate for those of the missing adhesins.
- Mesoporous carbon foam, synthesized via modified Pechini method, in a new dispersant of Salep as a novel substrate for electroanalytical determination of epinephrine in the presence of uric acid. [Journal Article]
- MSMater Sci Eng C Mater Biol Appl 2017 Jan 1; 70(Pt 1):544-551
- Mesoporous carbon foam (MCF) with particular properties was prepared via a simplistic and template-free procedure. The synthesized MCF was characterized by transmission electron microscopy, field emi...
Mesoporous carbon foam (MCF) with particular properties was prepared via a simplistic and template-free procedure. The synthesized MCF was characterized by transmission electron microscopy, field emission scanning electron microscopy, X-ray diffraction and BET surface area techniques. Porous MCF with pores diameters of 5 to 10nm provided a perfect substrate including extensive specific surface area to modify the electrodes surface. The obtained MCF was dispersed in Salep solution to prepare a stable suspension (S-MCF). The resultant composite was casted on the surface of glassy carbon electrode (GCE) to assemble the S-MCF modified GCE (S-MCF/GCE). Cyclic voltammetry (CV) was used to study the electrochemical behavior of epinephrine (Ep) and the determination of Ep was conducted by applying differential pulls voltammetry (DPV) in the presence of uric acid (UA). In the optimized conditions, the presented sensor is able to detect the concentration range of 0.1-12μM with a limit of detection of 40nM. The presented methodology possesses a reliable reproducibility, repeatability and stability in biological samples. These results proved that the S-MCF composite has a hopeful capacity in electrochemical sensors development.
- The Use of Epinephrine in Caudal Anesthesia Increases Stroke Volume and Cardiac Output in Children. [Journal Article]
- RAReg Anesth Pain Med 2016 Nov/Dec; 41(6):780-786
- CONCLUSIONS: Epinephrine added to local anesthetic injected for caudal anesthesia produces significant increases in SV, CO, and CI in children. Stroke volume and CI changes from epinephrine added to local anesthetic for caudal anesthesia seem to take place only in children 6 months or older.
- Stanniocalcin 1 Enhances Carbon Flux from Glucose to Lipids in White Retroperitoneal Adipose Tissue in the Fed Rat. [Journal Article]
- LLipids 2016; 51(11):1303-1307
- The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the ...
The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of (14)C from glucose into lipids in the rat WRAT. The increase in lipogenesis capacity supports the hypothesis that the activity of the glycerol-3-phosphate-generating pathway (glycolysis) from glucose is regulated by hSTC-1. The effect of hSTC-1 on de novo fatty acid synthesis and on glucose oxidation in WRAT is supported by an 85 % increase in (14)CO2 production from (14)C-glucose. The incubation of WRAT in the presence of hSTC-1 maintained the ADP/ATP ratio close to the control group. The presence of hSTC-1 in the incubation medium did not inhibit the lipolytic effect of epinephrine. In conclusion, hSTC-1 is one of the hormonal factors that control glucose metabolism in WRAT in the fed state.
- Serum serotonin as unexpected potential marker for staging of experimental hepatocellular carcinoma. [Journal Article]
- BPBiomed Pharmacother 2016; 83:407-411
- Hepatocellular carcinoma (HCC) is the primary cancer of the liver. The present study aimed to assess the potential role of the endogenous regulators of angiogenesis like neurotransmitters, as possibl...
Hepatocellular carcinoma (HCC) is the primary cancer of the liver. The present study aimed to assess the potential role of the endogenous regulators of angiogenesis like neurotransmitters, as possible HCC biomarkers. Five groups of rats were used in this study (8 rats per each): control healthy group (I), four intoxicated groups (II, III, IV, and V) used for induction of HCC with a single IP dose of diethylnitrosamine (DENA), 200mg/kg. Groups II, III, IV, and V were sacrificed after 8, 16, 24, and 32 weeks of DENA injection respectively. Serum levels of epinephrine, nor-epinephrine, serotonin, and dopamine of all animals were estimated using high performance liquid chromatography technique coupled with fluorescence detector (HPLC-FLD). Development of HCC was confirmed histopathologically. Our results showed a significant increase in 3 neurotransmitters (epinephrine, nor-epinephrine, and serotonin) in DENA intoxicated HCC rat model. Only serotonin exhibited a significant increase in early histological stage HCC development (16 weeks post DENA injection) in comparison to alpha-fetoprotein (AFP), (24 weeks post DENA injection). These results suggest that neurotransmitters (Epinephrine and Norepinephrine) may have a role as a biomarker for late histological stage HCC. Like AFP, while serotonin may be used for early stage HCC.
- Physical compatibility of tedizolid phosphate with selected i.v. drugs during simulated Y-site administration. [Journal Article]
- AJAm J Health Syst Pharm 2016 Nov 1; 73(21):1769-1776
- CONCLUSIONS: Tedizolid phosphate 0.8 mg/mL in 0.9% sodium chloride injection was physically compatible with 69 of 86 study drugs during simulated Y-site administration.
New Search Next
- Phenylethanolamine N-methyltransferase Gene Expression in Adrenergic Neurons of Spontaneously Hypertensive Rats. [Journal Article]
- NLNeurosci Lett 2016 Oct 18
- Epinephrine is synthesized by the catecholamine biosynthetic enzyme, phenylethanolamine N-methyltransferase (PNMT), primarily in chromaffin cells of the adrenal medulla and secondarily in brainstem a...
Epinephrine is synthesized by the catecholamine biosynthetic enzyme, phenylethanolamine N-methyltransferase (PNMT), primarily in chromaffin cells of the adrenal medulla and secondarily in brainstem adrenergic neurons of the medulla oblongata. Epinephrine is an important neurotransmitter/neurohormone involved in cardiovascular regulation; however, overproduction is detrimental with negative outcomes such as cellular damage, cardiovascular dysfunction, and hypertension. Genetic mapping studies have linked elevated expression of PNMT to hypertension. Adrenergic neurons are responsible for blood pressure regulation and are the only PNMT containing neurons in the brainstem. The purpose of the current study was to determine whether elevated blood pressure found in adult spontaneously hypertensive rats (SHR) is associated with altered regulation of the PNMT gene in catecholaminergic neurons. C1, C2, and C3 adrenergic regions of 16 week old Wistar Kyoto (WKY) and SHR rats were excised using micropunch microdissection for mRNA expression analyses. Results from the current study confirm high PNMT mRNA expression in all three brainstem adrenergic regions (C1: 2.96-fold; C2: 2.17-fold; C3 1.20-fold) of the SHR compared to normotensive WKY rats. Furthermore, the immediate early gene transcription factor (Egr-1) mRNA was elevated in the C1 (1.84-fold), C2 (8.57-fold) and C3 (2.41-fold) regions in the brainstem of the SHR. Low mRNA expression for transcription factors Sp1 and GR was observed, while no change was observed for AP-2. The findings presented propose that alterations in the PNMT gene regulation in the brainstem contribute to enhanced PNMT production and epinephrine synthesis in the SHR, a genetic model of hypertension.