(Infertility female) articles in PubMed
- Novel sperm and gonadotropin-releasing hormone-based recombinant fusion protein: Achievement of 100% contraceptive efficacy by co-immunization of male and female mice. [Journal Article]
- Mol Reprod Dev 2016 Sep 27MR
- Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino ...
Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17N or TT-KK-Sp17C , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli. Immunization of female FVB/J mice, using alum as an adjuvant, led to the generation of high antibody titres against the above proteins. Antibodies against both N- and C-terminal fragments of Sp17 reacted with the entire capacitated mouse spermatozoa, whereas those against equatorin reacted exclusively with the equatorial region. Despite the reactivity of all immune sera, only sera from mice immunized with TT-KK-Sp17N and TT-KK-Sp17C significantly reduced mouse in vitro fertilization. Mating studies of the immunized females with un-immunized male mice revealed the highest infertility in the TT-KK-Sp17C -immunized group. In an attempt to further boost the immune response, the C-terminal fragment of Sp17 was expressed as fusion protein with a tandem repeat of gonadotropin-releasing hormone (GnRH) (Sp17C -GnRH2 ). Immunization of both male and female mice with Sp17C -GnRH2 led to higher contraceptive efficacy compared to mice immunized with TT-KK-Sp17C . Interestingly, mating studies wherein partners were both immunized with Sp17C -GnRH2 showed a complete failure of female mice to conceive. Thus, immunization of both males and females with Sp17C -GnRH2 has the potential to increase contraceptive efficacy. This article is protected by copyright. All rights reserved.
- Videovaginoscopy of the canine vagina. [Journal Article]
- Reprod Domest Anim 2016; 51 Suppl 1:31-36RD
- Endoscopy enables a rapid and non-traumatic examination of the vaginal cavity of female dogs. Its implementation requires specific equipment as well as knowledge about the species-specific vaginal an...
Endoscopy enables a rapid and non-traumatic examination of the vaginal cavity of female dogs. Its implementation requires specific equipment as well as knowledge about the species-specific vaginal anatomy and the procedure. Vaginoscopy is the diagnostic tool of choice for several vaginal conditions and diseases such as congenital abnormalities, neoplasia or ectopic ureter. Furthermore, vaginal inspection may provide additional information in cases of vulvar discharge or infertility. Vaginoscopy is a superior tool compared to other methods in assisted reproduction. Changes of the vaginal mucosa during the oestrous cycle can be observed to determine the hormonal actions and the time of ovulations in addition to other routine examinations, such as progesterone assay and vaginal cytology. Finally, intrauterine insemination, which is recommended for insemination with frozen-thawed semen, can be performed endoscopically by transcervical catheterization.
- Complex Laparoscopic Myomectomy with Severe Adhesions Performed with Proper Preventive Measures and Power Morcellation Provides a Safe Choice in Certain Infertility Cases. [Journal Article]
- Case Rep Obstet Gynecol 2016; 2016:4705790CR
- Laparoscopic myomectomy offers a real benefit to infertile patients with uterine fibroids and peritoneal adhesions. The procedure requires a skilled surgeon and laparoscopy technique to minimize adhe...
Laparoscopic myomectomy offers a real benefit to infertile patients with uterine fibroids and peritoneal adhesions. The procedure requires a skilled surgeon and laparoscopy technique to minimize adhesion formation and other proven benefits. Restrictions arise since this procedure requires power morcellation for fibroid tissue extraction. Two years ago, the Food and Drug Administration in the United States of America (FDA) issued the alert on power morcellation for uterine leiomyomas, addressing the risk of malignant cell spreading within the abdominal cavity (actual risk assessment from 1 in 360 to 1 in 7400 cases). We review a 30-year-old female, without previous gestations, hypermenorrhea, intermenstrual bleeding, and chronic pelvic pain. Transvaginal ultrasound reports multiple fibroids in the right portion of a bicornuate uterus. Relevant history includes open myomectomy 6 years before and a complicated appendectomy, developing peritonitis within a year. Laparoscopy revealed multiple adhesions blocking uterine access, a bicornuate uterus, and myomas in the expected site. Myomectomy was performed utilizing power morcellation with good results. FDA recommendations have diminished this procedure's selection, converting many to open variants. This particular case was technically challenging, requiring morcellation, and safety device deployment was impossible, yet the infertility issue was properly addressed. Patient evaluation, safety measures, and laparoscopy benefits may outweigh the risks in particular cases as this one.
- Female cancer survivors exposed to alkylating-agent chemotherapy have unique reproductive hormone profiles. [Journal Article]
- Fertil Steril 2016 Sep 22FS
- CONCLUSIONS: Women exposed to alkylating agents have a unique reproductive hormone milieu that is not solely explained by age or ovarian reserve. The urinary hormone profile observed in survivors appears more similar to same-age control subjects than to late-reproductive-age women with similar ovarian reserve, which may suggest that age plays a more important role than ovarian reserve in the follicular dynamics of survivors.
- Population trends and live birth rates associated with common ART treatment strategies. [Journal Article]
- Hum Reprod 2016 Sep 22HR
- CONCLUSIONS: There were striking improvements in per-cycle live birth rates observed for frozen/thaw embryo transfers, blastocyst transfer and single embryo transfer (SET), while live birth rates following ICSI were lower than IVF for non-male factor infertility in most years.This is a retrospective cohort analysis and cannot confirm causality. High-level evidence on the effectiveness of particular ART techniques, particularly ICSI and blastocyst culture, requires prospective randomized controlled trials or detailed statistical analysis using large-scale data that counts for fertilization failure, embryo loss, prognostic factors and cycle characteristics.The most striking improvements in ART success rates in Australia have been observed for frozen/thaw embryo transfers, blastocyst transfer and SET. Further studies of the role of ICSI in non-male factor infertility and blastocyst transfer success rates that take into account embryo loss are needed.
- Decreased Sperm Motility Retarded ICSI Fertilization Rate in Severe Oligozoospermia but Good-Quality Embryo Transfer Had Achieved the Prospective Clinical Outcomes. [Journal Article]
- PLoS One 2016; 11(9):e0163524Plos
- CONCLUSIONS: The reduction of motile spermatozoa in severe oligozoospermia decreased the rates of fertilization and good-quality embryo. Obtaining and transfer of good-quality embryos was the good prognostic to achieve prospective clinical outcomes regardless of the severity of oligozoospermia.
- Fertility and sexual function: a gap in training in pediatric endocrinology. [Journal Article]
- J Pediatr Endocrinol Metab 2016 Sep 22JP
- CONCLUSIONS: Infertility and sexual dysfunction are often overlooked in pediatric care. Our results suggest that pediatric endocrinologists, who frequently manage male and female hypogonadism, should also receive formal training in these areas. Optimizing counseling would help prevent missed opportunities for fertility preservation and alleviate distress among patients and families.
- Comparison of the Effect of Clomiphene- Estradiol Valerate vs Letrozole on Endometrial Thickness, Abortion and Pregnancy Rate in Infertile Women with Polycystic Ovarian Syndrome. [Journal Article]
- J Clin Diagn Res 2016; 10(8):QC10-3JC
- CONCLUSIONS: Letrozole increased endometrial thickness and pregnancy rate in infertile women, therefore its administration is recommended.
- Fibroblast growth factor 21 has no direct role in regulating fertility in female mice. [Journal Article]
- Mol Metab 2016; 5(8):690-8MM
- CONCLUSIONS: We conclude that FGF21 is not a direct physiological regulator of fertility in mice. The infertility observed in FGF21 overexpressing mice is likely driven by the increased energy expenditure and consequent excess calorie requirements resulting from high FGF21 levels.
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- Hysterolaparoscopy in the Evaluation and Management of Female Infertility. [Journal Article]
- J Obstet Gynaecol India 2016; 66(Suppl 1):478-81JO
- CONCLUSIONS: Diagnostic hysterolaparoscopy is an effective diagnostic and therapeutic modality for certain significant and correctable abnormalities in pelvis, tubes and uterus which are missed by other imaging modalities.