(TMP) articles in PubMed
- Pharmacoepigenetics of the role of DNA methylation in μ-opioid receptor expression in different human brain regions. [Journal Article]
- Epigenomics 2016 Sep 29E
- CONCLUSIONS: While high or low μ-opioid receptor expression significantly correlated with local OPRM1 mRNA levels, there was no corresponding association with OPRM1 methylation status. Additional experiments in human cell lines showed that changes in DNA methylation associated with changes in μ-opioid expression were an order of magnitude greater than differences in brain. Hence, different degrees of DNA methylation associated with chronic opioid exposure are unlikely to exert a major role in the region-specificity of μ-opioid-receptor expression in the human brain.
- Brightly Luminescent and Kinetically Inert Lanthanide Bioprobes Based on Linear and Pre-organized Chelators. [Journal Article]
- Bioconjug Chem 2016 Sep 29BC
- The synthesis, photophysical properties and kinetic stability of a series of water-soluble, highly emissive Tb(III) and Eu(III) complexes featuring triethylenetetraamine hexaacetic acid (TTHA) and cy...
The synthesis, photophysical properties and kinetic stability of a series of water-soluble, highly emissive Tb(III) and Eu(III) complexes featuring triethylenetetraamine hexaacetic acid (TTHA) and cyclohexyl triethylenetetraamine hexaacetic acid (cyTTHA) chelator scaffolds and carbostyril sensitizers are reported. The unique and modular design of the chelators gives rise to striking quantum yields of emission in aqueous solutions (up to 54%) as well as the characteristic lanthanides' photophysical properties (long excited state lifetimes, large effective Stokes shifts, and narrow emission peaks). Furthermore, the pre-organized chelators (L3, L4 and L6) bind metal within minutes at ambient temperature yet exhibit substantial resistance to transchelation in the presence of a challenge solution (EDTA, 1 mM). Moreover, the Eu(III) complex of L4 remains stably luminescent in HeLa cells over hours, demonstrating the suitability of these compounds for live cell imaging applications. Representative chelators suitable for derivatization and protein bioconjugation were also prepared that were functionalized with clickable azide and alkyne moieties, biotin and trimethoprim (TMP). With exceptional long-wavelength brightness, enhanced kinetic inertness, and an adaptable synthetic route, the reported lanthanide complexes are promising probes and labels for time-gated bioanalysis, biosensing and optical microscopy.
- Progress does not just come in giant leaps: adapting techniques for the study of inflammation to novel applications. [Journal Article]
- Inflamm Res 2016 Sep 28IR
- CONCLUSIONS: Fine tuning of experimental approaches and evaluation technologies can often still provide innovative, clinically relevant insights into the potential beneficial effects of drugs and pharmacological agents.
- Synthesis of a Sterically Demanding Dispiropiperidine and Its Application in Monoamidodialkyl Zincate Complexes. [Journal Article]
- Inorg Chem 2016 Sep 28IC
- The new sterically hindered piperidine analog, dispiro[cyclohexane-2,2'-piperidine-6',2″-cyclohexane] (CPC(H), 2), and its N-methylated derivative CPC(Me) (3) were synthesized from commercially avail...
The new sterically hindered piperidine analog, dispiro[cyclohexane-2,2'-piperidine-6',2″-cyclohexane] (CPC(H), 2), and its N-methylated derivative CPC(Me) (3) were synthesized from commercially available starting materials in short steps. The N-lithiated amide LiCPC (4) was also isolated from 2 as a cyclictrimer in single crystals and showed slightly larger steric hindrance than that of lithium 2,2,6,6-tetramethylpiperidide (LiTMP) in the competitive methylation reaction with methyl trifluoromethanesulfonate. In addition, the heterobimetallic heteroleptic zincate complexes [Li(μ-NR2)(μ-Et)Zn(Et)] (NR2 = CPC, 5, and NR2 = TMP, 6) were obtained as THF- and TMEDA-coordinated monomer 5·(THF)2, 6·(THF)2, 5·TMEDA, and 6·TMEDA (THF = tetrahydrofuran, TMEDA = N,N,N',N'-tetramethylethylenediamine). These molecular structures bearing different amido ligands in single crystals showed little structural differences from crystallographic studies. Diffusion-ordered spectroscopy (DOSY) revealed that the solution structures of the zincate complexes 5·(THF)2 and 6·(THF)2 only differ in the number of coordination THF molecules. In the deprotonation reactions with tert-butyl 3-bromobenzoate, the zincate complexes containing the CPC ligand [Li(μ-CPC)(μ-R)Zn(R)] (R = Et (5), tBu) showed moderately improved regioselectivity for the 6 position in comparison to those containing the TMP ligand [Li(μ-TMP)(μ-R)Zn(R)] (R = Et (6), tBu).
- Tetramethylpyrazine and astragaloside IV synergistically ameliorate left ventricular remodeling and preserve cardiac function in a rat myocardial infarction model. [Journal Article]
- J Cardiovasc Pharmacol 2016 Sep 20JC
- Tetramethylpyrazine (TMP) and astragaloside IV (AGS-IV) are herbal ingredients that have been demonstrated in animal models to limit infarct size and protect cardiomyocytes in the acute phase of myoc...
Tetramethylpyrazine (TMP) and astragaloside IV (AGS-IV) are herbal ingredients that have been demonstrated in animal models to limit infarct size and protect cardiomyocytes in the acute phase of myocardial infarction (MI), yet their long-term cardio-protective effects have not been evaluated. In the present study, TMP and/or ASG-IV were administrated to rats for 14 days after MI. Echocardiography revealed that the left ventricular (LV) dimensions and cardiac function was preserved in the MI rats with TMP and ASG-IV treatment, compared to untreated MI rats. Moreover, the LV dimensions and cardiac function in the MI rats with TMP and ASG-IV co-treatment were comparable to the sham-operated rats. In addition, TMP and ASG-IV synergistically inhibited LV fibrosis by attenuating MI-induced collagen deposition and elevation of transforming growth factor β1. TMP and ASG-IV, alone or in synergy, enhanced angiogenesis in the infarcted myocardium and reduced cardiac hypertrophy of the remote myocardium following MI. Furthermore, TMP and ASG-IV mutually upregulated the expression of Sonic hedgehog (Shh), Smmothened (Smo) and Glioblastoma-2 (Gli2), the receptor and signal transducer of Shh signaling pathway, in the infarcted myocardium. In summary, in the circumstance of the irreversible ischemic injury, the anti-fibrotic and pro-angiogenic properties of TMP and ASG-IV on the non-affected tissues contributes to the cardio-protection in the healing phase post MI, and the cardio-protective effects are likely to be mediated via the Shh pathway.
- Harvesting microalgal biomass using crossflow membrane filtration: Critical flux, filtration performance and fouling characterization. [Journal Article]
- Environ Technol 2016 Sep 26; :1-33ET
- The purpose of this study was to investigate the efficient harvesting of microalga biomass through crossflow membrane filtration. The microalga biomass harvesting experiments were performed using one...
The purpose of this study was to investigate the efficient harvesting of microalga biomass through crossflow membrane filtration. The microalga biomass harvesting experiments were performed using one microfiltration membrane (pore size: 0.2 µm, made from polyvinylidene fluoride) and three ultrafiltration membranes (molecular weight cut-off: 150, 50, and 30 kDa, made from polyethersulfone, hydrophilic polyethersulfone, and regenerated cellulose, respectively). Initially, to minimize membrane fouling caused by microalga cells, experiments with the objective of determining the critical flux were performed. Based on the critical flux calculations, the best performing membrane was confirmed to be the UH050 membrane, produced from hydrophilic polyethersulfone material. Furthermore, we also evaluated the effect of transmembrane pressure and crossflow velocity on filtration flux. It was observed that membrane fouling was affected not only by the membrane characteristics, but also by the transmembrane pressure and crossflow velocity. In all membranes, it was observed that increasing crossflow velocity was associated with increasing filtration flux, independent of the transmembrane pressure.
- Effects of ozone pretreatment and operating conditions on membrane fouling behaviors of an anoxic-aerobic membrane bioreactor for oil sands process-affected water (OSPW) treatment. [Journal Article]
- Water Res 2016 Sep 9; 105:444-455WR
- Two identical anoxic-aerobic membrane bioreactors (MBRs) were operated in parallel for 742 consecutive days for raw and ozonated oil sands process-affected water (OSPW) treatment. The MBRs not only s...
Two identical anoxic-aerobic membrane bioreactors (MBRs) were operated in parallel for 742 consecutive days for raw and ozonated oil sands process-affected water (OSPW) treatment. The MBRs not only substantially degraded OSPW classical and oxidized NAs, but also demonstrated outstanding membrane fouling control performance (the MBR receiving raw OSPW experienced its first severe fouling with a transmembrane pressure (TMP) of -35 kPa on Day 433). The mild ozonation (30 mg O3/L) pretreatment of OSPW remarkably enhanced OSPW NA degradation, and improved the MBR's fouling control in terms of prolonged long-term slow TMP growth phase and reduced TMP jump frequency. Ozonation substantially altered the feed water organic composition, reshaped the microbial community (e.g., reduced growth of extracellular polymeric substances (EPS) producers and biofilm formation facilitators), and lowered EPS production and EPS protein/polysaccharides (PN/PS) ratio, consequently leading to the better fouling control. Examination on fouling behaviors at different HRTs of the raw OSPW MBR indicates that HRT also played a role in determining the dominating fouling mechanism during the sharp TMP rise phase. Therefore, the results of this study suggest that the low-dose ozone pretreatment is a good option to enhance organic contaminant degradation and alleviate membrane fouling in the MBR for OSPW treatment.
- Multiscale correlations of iron phases and heavy metals in technogenic magnetic particles from contaminated soils. [Journal Article]
- Environ Pollut 2016 Sep 20; 219:19-27EP
- Technogenic magnetic particles (TMPs) are carriers of heavy metals and organic contaminants, which derived from anthropogenic activities. However, little information on the relationship between heavy...
Technogenic magnetic particles (TMPs) are carriers of heavy metals and organic contaminants, which derived from anthropogenic activities. However, little information on the relationship between heavy metals and TMP carrier phases at the micrometer scale is available. This study determined the distribution and association of heavy metals and magnetic phases in TMPs in three contaminated soils at the micrometer scale using micro-X-ray fluorescence (μ-XRF) and micro-X-ray absorption near-edge structure (μ-XANES) spectroscopy. Multiscale correlations of heavy metals in TMPs were elucidated using wavelet transform analysis. μ-XRF mapping showed that Fe was enriched and closely correlated with Co, Cr, and Pb in TMPs from steel industrial areas. Fluorescence mapping and wavelet analysis showed that ferroalloy was a major magnetic signature and heavy metal carrier in TMPs, because most heavy metals were highly associated with ferroalloy at all size scales. Multiscale analysis revealed that heavy metals in the TMPs were from multiple sources. Iron K-edge μ-XANES spectra revealed that metallic iron, ferroalloy, and magnetite were the main iron magnetic phases in the TMPs. The relative percentage of these magnetic phases depended on their emission sources. Heatmap analysis revealed that Co, Pb, Cu, Cr, and Ni were mainly derived from ferroalloy particles, while As was derived from both ferroalloy and metallic iron phases. Our results indicated the scale-dependent correlations of magnetic phases and heavy metals in TMPs. The combination of synchrotron based X-ray microprobe techniques and multiscale analysis provides a powerful tool for identifying the magnetic phases from different sources and quantifying the association of iron phases and heavy metals at micrometer scale.
- Remineralizing potential of a 60-s in vitro application of Tooth Mousse Plus. [Journal Article]
- Int J Paediatr Dent 2016 Sep 23IJ
- CONCLUSIONS: TMP applied for 60 s significantly remineralized the artificial carious lesions. No additional benefit was evident when TMP was preceded by treatment with 1000 ppm F dentifrice.
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- [Etiology and drug resistance of non-typhi Salmonella in Henan province, 2011-2015]. [Journal Article]
- Zhonghua Liu Xing Bing Xue Za Zhi 2016 Sep 10; 37(9):1253-1256ZL
- Objective: To analyze the serotypes and antibiotic resistance phenotypes of non-typhi Salmonella strains in Henan province from 2011 to 2015. Methods: The stool samples were collected from diarrhea p...
Objective: To analyze the serotypes and antibiotic resistance phenotypes of non-typhi Salmonella strains in Henan province from 2011 to 2015. Methods: The stool samples were collected from diarrhea patients, and enriched with SBG enrichment broth and the pathogen isolation was conducted with CHROMAgar selective culture medium at 37 ℃ for 18-24 hours using KIA/MIU biochemical action and API20E biochemical system slab to identify Salmonella strains. The serotypes of all the positive strains were detected with SSI Salmonella typing sera. According to K-B drug susceptibility testing method published by USA clinical and Laboratory Standards Institute (CLSI), the antibiotics resistant phenotype of the positive strains were analyzed. Results: A total of 1 351 strains of non-typhi Salmonella were isolated, in which 811 were from males, 540 were from females. The ratio of men to women was 1.5∶1. Children and young adults were mainly affected. The pathogen isolation was mainly in May-October during a year. The 1 351 strains of non-typhi Salmonlla were divided into 58 serotypes. S. enteritidis, S. typhimurium, S. agona, S. derby, S. indiana, S. senftenberg and S. thompson ranked 1(st)-7(th). The drug-resistance rate of the 1 351 strains was 46.1% to synthetic broad-spectrum penicillin ampicillin (AMP), 19.5% and 21.2% to the three generation cephalosporin ceftazidime (CAZ) and cefotaxime (CTX), 8.8% to the four generation cephalosporins cefepime (FEP), 58.7% to the one generation of quinolones nalidixic acid (NAL), 14.7% to the three generation fluoroquinolone ciprofloxacin (CIP) and norfloxacin (NOR), 25.0% and 35.6% to aminoglycoside antibiotic gentamicin (GEN) and streptomycin (STR), 35.4% to amphenicols (CHL), 31.7% to sulfonamide trimethoprim (TMP) and 37.8% to tetracycline (TET). Totally 879 strains were multidrug resistant (65.1%): 350 strains were resistant to 3-4 kinds of antibiotics (25.9%), 309 strains were resistant to 5-7 kinds of antibiotics (22.9%), 174 strains were resistant to 8-10 kinds of antibiotics (12.9%) and 48 strains were resistant to 11-12 kinds of antibiotics (3.6%). Conclusion: The serotypes of non-typhi Salmonella strains isolated from Henan province varied, some strains were resistant to 12 kinds of antibiotics commonly used in clinical treatment and the multidrug resistance has become serious.