(albumin human ) articles in PubMed
- Antifungal prophylaxis of patients undergoing allogenetic hematopoietic stem cell transplantation in China: a multicenter prospective observational study. [Journal Article]
- J Hematol Oncol 2016; 9(1):97JH
- CONCLUSIONS: This observational study indicates that prophylaxis of IFD is helpful to reduce the incidence of IFDs and improve the OS of patients after allo-HSCT.
- Aberrant Co-Localization of Synaptic Proteins Promoted by Alzheimer's Disease Amyloid-β Peptides: Protective Effect of Human Serum Albumin. [Journal Article]
- J Alzheimers Dis 2016 Sep 17JA
- Amyloid-β (Aβ), Aβ40, Aβ42, and, recently, Aβ25-35 have been directly implicated in the pathogenesis of Alzheimer's disease. We have studied the effects of Aβ on neuronal death, reactive oxygen speci...
Amyloid-β (Aβ), Aβ40, Aβ42, and, recently, Aβ25-35 have been directly implicated in the pathogenesis of Alzheimer's disease. We have studied the effects of Aβ on neuronal death, reactive oxygen species (ROS) production, and synaptic assembling in neurons in primary culture. Aβ25-35, Aβ40, and Aβ42 significantly decreased neuronal viability, although Aβ25-35 showed a higher effect. Aβ25-35 showed a more penetrating ability to reach mitochondria while Aβ40 did not enter the neuronal cytosol and Aβ42 was scarcely internalized. We did not observe a direct correlation between ROS production and cell death because both Aβ40 and Aβ42 decreased neuronal viability but Aβ40 did not change ROS production. Rather, ROS production seems to correlate with the penetrating ability of each Aβ. No significant differences were found between Aβ40 and Aβ42 regarding the extent of the deleterious effects of both peptides on neuronal viability or synaptophysin expression. However, Aβ40 elicited a clear delocalization of PSD-95 and synaptotagmin from prospective synapsis to the neuronal soma, suggesting the occurrence of a crucial effect of Aβ40 on synaptic disassembling. The formation of Aβ40- or Aβ42-serum albumin complexes avoided the effects of these peptides on neuronal viability, synaptophysin expression, and PSD-95/synaptotagmin disarrangement suggesting that sequestration of Aβ by albumin prevents deleterious effects of these peptides. We can conclude that Aβ borne by albumin can be safely transported through body fluids, a fact that may be compulsory for Aβ disposal by peripheral tissues.
- Copolymer Brushes with Temperature-triggered, Reversibly Switchable Bactericidal and Antifouling Properties for Biomaterial Surfaces. [Journal Article]
- ACS Appl Mater Interfaces 2016 Sep 23AA
- The adherence of bacteria and the formation of biofilm on implants is a serious problem that often leads to implant failure. A series of antimicrobial coatings have been constructed to resist bacteri...
The adherence of bacteria and the formation of biofilm on implants is a serious problem that often leads to implant failure. A series of antimicrobial coatings have been constructed to resist bacterial adherence or to kill bacteria through contact with or release of antibacterial agents. The accumulation of dead bacteria facilitates further bacterial contamination and biofilm development. Herein, we have designed and constructed a novel, reversibly switchable bactericidal and antifouling surface through surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization to combine thermally responsive N-isopropylacrylamide (NIPAAm) and bactericidal quaternary ammonium salts (2-(dimethylamino)-ethyl methacrylate (DMAEMA+)). Measurements of spectroscopic ellipsometry, water contact angle and X-ray photoelectron spectroscopy were used to examine the process of the surface functionalization. The temperature-responsive P(DMAEMA+-co-NIPAAm) copolymer coating can switch by phase transition between a hydrophobic capturing surface at high temperature and a relatively hydrophilic antifouling surface at lower temperature. The quaternary ammonium salts of PDMAEMA+ displayed bactericidal efficiency against both Escherichia coli and Staphylococcus aureus. The functionalized surface could efficiently prevent bovine serum albumin adsorption and had good biocompatibility against human lens epithelial cells.
- Adult-derived human liver stem/progenitor cells infused 3 days post-surgery improve liver regeneration in a mouse model of extended hepatectomy. [Journal Article]
- Cell Transplant 2016 Sep 21CT
- There is growing evidence that cell therapy constitutes a promising strategy for liver regenerative medicine. In the setting of hepatic cancer treatments, cell therapy could prove a useful therapeuti...
There is growing evidence that cell therapy constitutes a promising strategy for liver regenerative medicine. In the setting of hepatic cancer treatments, cell therapy could prove a useful therapeutic approach for managing the acute liver failure that occurs following extended hepatectomy. In this study, we examined the influence of delivering adult human liver mesenchymal stem/progenitor cells (ADHLSCs) at two different early time points in an immunodeficient mouse model (Rag(2-/-)IL2Rγ(-/-) ) that had undergone a 70%-hepatectomy procedure. The hepato-mesenchymal cells were intrasplenically infused either immediately after surgery (n= 26) or following a critical 3-day period (n=26). We evaluated the cells' capacity to engraft at Day 1 and Day 7 following transplantation by means of human Alu qPCR quantification, along with histological assessment of human albumin and alpha-smooth muscle actin. In addition, cell proliferation (anti-mouse and human Ki67 staining) and murine liver weight were measured in order to evaluate liver regeneration. At Day 1 post-transplantation, the ratio of human to mouse cells was similar in both groups, whereas 1 week post-transplantation, this ratio was significantly improved (p <0.016) in mice receiving ADHLSC injection at Day 3 post-hepatectomy (1.7%), compared to those injected at the time of surgery (1%). Based on liver weight, mouse liver regeneration was more extensive 1 week post-transplantation in mice transplanted with ADHLSCs (+65.3%) compared to that of mice from the sham vehicle group (+42.7%). In conclusion, infusing ADHLSCs 3 days after extensive hepatectomy improves the cell engraftment and murine hepatic tissue regeneration, thereby confirming that ADHLSCs could be a promising cell source for liver cell therapy and hepatic tissue repair.
- Detection of Carbofuran-Protein Adducts in Serum of Occupationally Exposed Pesticide Factory Workers in Pakistan. [Journal Article]
- Chem Res Toxicol 2016 Sep 22CR
- This study was conducted to investigate the protein adducts with pesticides in a cohort of 172 factory workers that were exposed to a mixture of pesticides. The 35 samples showing considerable variat...
This study was conducted to investigate the protein adducts with pesticides in a cohort of 172 factory workers that were exposed to a mixture of pesticides. The 35 samples showing considerable variation in biochemical parameters i.e. ButyrylCholinestrase (BChE), Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT), Gamma-GlutamylTransferase (GGT), Serum Glutamic Pyruvic Transaminase (SGPT), Alkaline Phosphatase (ALP/ALKP), Lactate Dehydrogenase (LDH), and Creatine Phosphokinase (CPK) enzymes and controls were analyzed by reversed-phase nano-scale liquid chromatography tandem mass spectrometry (nLC-MS/MS) on an Orbitrap mass spectrometer employing a shotgun proteomics approach. Only protein adducts with carbofuran were found on serum proteins of these workers. These adducts were of carbofuran labeled lysine (Lys-142, Lys--183, Lys-287, Lys-467), Arginine (Arg-210, Arg-242, Arg-256) from serum albumin and serine (Ser-07, Ser-54, Ser-150) from immunoglobulin proteins. The Arginine residues (Arg-210, Arg-242, Arg-246, Arg-434) from albumin were also found to be glycated in sera of workers showing a high level of glucose who also had glycated arginine (Arg-1120) modified with carbofuran in their tankyrase-1-binding protein. The number of tandem mass spectra of modified peptides increased with increasing time of exposure. This is the first report to demonstrate the presence of carbofuran-labeled albumin, immunoglobulin and glycated arginine which concludes that lysine and arginine of human albumin and serine of immunoglobulin are covalently modified in the serum of workers that were occupationally exposed to carbofuran and the modification is detectable by tandem mass spectrometry. These peptides modified with carbofuran can potentially be used as a biomarker of carbofuran exposure.
- Effect of Processing Delay and Storage Conditions on Urine Albumin-to-Creatinine Ratio. [Journal Article]
- Clin J Am Soc Nephrol 2016 Sep 21CJ
- CONCLUSIONS: Mailed urine samples collected with preservative and received within 7 days if ambient temperature is ≤18°C, or within 2 days if the temperature is higher but does not exceed 30°C, are suitable for the measurement of urine albumin-to-creatinine ratio in randomized trials. Preserved samples frozen to -40°C or -80°C for 6 months before analysis also seem suitable.
- Lack of R-Ras Leads to Increased Vascular Permeability in Ischemic Retinopathy. [Journal Article]
- Invest Ophthalmol Vis Sci 2016 Sep 1; 57(11):4898-4909IO
- CONCLUSIONS: Our results suggest that R-Ras has a role in controlling retinal vessel maturation and stabilization in ischemic retinopathy and provides a potential target for pharmacologic manipulation to treat diabetic retinopathy.
- A Multi-Laboratory, Multi-Country Study to Determine Minimal Inhibitory Concentration Quality Control Ranges for PhenotypicDrug-Susceptibility Testing of Selected First-Line Anti-TB Drugs, Second-Line Injectables, Fluoroquinolones, Clofazimine and Linezolid. [Journal Article]
- J Clin Microbiol 2016 Sep 21JC
- CONCLUSIONS: Using strict CLSI criteria, QC ranges against the MTB H37Rv reference strain were established for the majority of commonly used antituberculosis drugs, with a convenient 7H9 broth microdilution MIC method suitable for use in resource-limited settings.
- Factors associated with mortality among patients with active pulmonary tuberculosis requiring intensive care. [Journal Article]
- Singapore Med J 2016 Sep 22SM
- CONCLUSIONS: In-hospital mortality from active PTB requiring ICU admission was 62.7% and low albumin was an independent predictor of mortality in this study.
New Search Next
- Investigating the Structural Change in Protein Aqueous Solution using Temperature-Dependent Near-Infrared Spectroscopy and Continuous Wavelet Transform. [Journal Article]
- Appl Spectrosc 2016 Sep 20AS
- The circulatory protein, human serum albumin (HSA), is widely used as a model protein for the study of protein structure. In this work, the structures of human serum albumin in aqueous solutions are ...
The circulatory protein, human serum albumin (HSA), is widely used as a model protein for the study of protein structure. In this work, the structures of human serum albumin in aqueous solutions are studied using temperature-dependent near-infrared (NIR) spectroscopy with the aid of continuous wavelet transform (CWT). Near-infrared spectra of human serum albumin solutions with different concentrations were measured over a temperature range of 30-85 ℃. Then, continuous wavelet transform was performed on the spectra to enhance the resolution. As a result of the resolution enhancement, spectral bands around 4361, 4521, 4600 and 4260 cm(-1) were extracted from the overlapping low-resolution signals. The four bands can be assigned to the protein structures of α-helix, β-sheet, an intermediate state and side chains, respectively. The variations in intensity of the bands around 4361 and 4521 cm(-1) with temperature show that the increase of temperature leads to the loss of α-helical structure but the formation of β-sheet, and the denaturation temperature of human serum albumin is about 55 ℃. The variation of the band around 4600 cm(-1) indicates that the temperature-induced unfolding process of human serum albumin occurs through a stable intermediate state, and a significant change in the microenvironment of the side chains about 63 ℃ is observed from the variation of the band around 4260 cm(-1) On the other hand, the transformed spectra in the region of 8000-5600 cm(-1) provide an explicit evidence for the structural changes of water during the process of protein denaturation, and the unfolding process of HSA can be reflected by these changes.