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Stool fat, fecal fat stain.
To assess for the presence of fat in the stool toward diagnosing malabsorption disorders such as Crohn’s disease and cystic fibrosis.
Stool (80 mL) aliquot from an unpreserved and homogenized 24- to 72-hr timed collection. Random specimens may also be submitted.
(Method: Stain with Sudan black or oil red O. Treatment with ethanol identifies neutral fats; treatment with acetic acid identifies fatty acids.)
|Neutral fat||Less than 60 fat globules/hpf*|
|Fatty acids||Less than 100 fat globules/hpf|
|Age (normal diet)|
|Infant (breast milk)||Less than 1 g/24 hr|
|0–6 yr||Less than 2 g/24 hr|
|Adult||2–7 g/24 hr; less than 20% of total solids|
|Adult (fat-free diet)||Less than 4 g/24 hr|
|*hpf = high-power field.|