| 5-Hydroxyindoleacetic AcidNursing Implications Procedure  Pretest - Positively identify the patient using at least two unique identifiers before providing care, treatment, or services.
- Patient Teaching: Inform the patient this test can assist in diagnosing tumor.
- Obtain a history of the patient’s complaints, including a list of known allergens, especially allergies or sensitivities to latex.
- Obtain a history of the patient’s endocrine, gastrointestinal, and immune systems; symptoms; and results of previously performed laboratory tests and diagnostic and surgical procedures.
- Obtain a list of the patient’s current medications, including herbs, nutritional supplements, and nutraceuticals (see Effects of Natural Products on Laboratory Values).
- Review the procedure with the patient. Provide a nonmetallic urinal, bedpan, or toilet-mounted collection device. Address concerns about pain and explain to the patient that there should be no discomfort during the procedure.
- Inform the patient that all urine collected over a 24-hr period must be saved; if a preservative has been added to the container, instruct the patient not to discard the preservative. Instruct the patient not to void directly into the container. Instruct the patient to avoid defecating in the collection device and to keep toilet tissue out of the collection device to prevent contamination of the specimen. Place a sign in the bathroom as a reminder to save all urine.
- Instruct the patient to void all urine into the collection device, then pour the urine into the laboratory collection container. Alternatively, the specimen can be left in the collection device for a health-care staff member to add to the laboratory collection container.
- Sensitivity to social and cultural issues, as well as concern for modesty, is important in providing psychological support before, during, and after the procedure.
- There are no fluid restrictions unless by medical direction.
- Inform the patient that foods and medications listed under “Interfering Factors” should be restricted by medical direction for at least 4 days before specimen collection.
Intratest - Ensure that the patient has complied with dietary and medication restrictions; assure foods and medications listed under “Interfering Factors” have been restricted for at least 4 days prior to the procedure.
- If the patient has a history of allergic reaction to latex, avoid the use of equipment containing latex.
- Instruct the patient to cooperate fully and to follow directions.
- Observe standard precautions, and follow the general guidelines in Patient Preparation and Specimen Collection. Positively identify the patient, and label the appropriate specimen container with the corresponding patient demographics, initials of the person collecting the specimen, date, and time of collection.
Timed Specimen- Obtain a clean 3-L urine specimen container, toilet-mounted collection device, and plastic bag (for transport of the specimen container). The specimen must be refrigerated or kept on ice throughout the entire collection period. If an indwelling urinary catheter is in place, the drainage bag must be kept on ice.
- Begin the test between 6 and 8 a.m. if possible. Collect first voiding and discard. Record the time the specimen was discarded as the beginning of the timed collection period. The next morning, ask the patient to void at the same time the collection was started, and add this last voiding to the container. Urinary output should be recorded throughout the collection time.
- If an indwelling catheter is in place, replace the tubing and container system at the start of the collection time. Keep the container system on ice during the collection period, or empty the urine into a larger container periodically during the collection period; monitor to ensure continued drainage. Conclude the test the next morning at the same hour the collection was begun.
- At the conclusion of the test, compare the quantity of urine with the urinary output record for the collection; if the specimen contains less than what was recorded as output, some urine may have been discarded, invalidating the test.
- Include on the specimen collection container’s label the amount of urine, test start and stop times, and ingestion of any foods or medications that can affect test results. Promptly transport the specimen to the laboratory for processing and analysis.
Post Test - A report of the results will be made available to the requesting health-care provider (HCP), who will discuss the results with the patient.
- Instruct the patient to resume usual diet, as directed by the HCP. Consideration may be given to niacin supplementation and increased protein, if appropriate, for patients with abnormal findings. In some cases, the tumor may divert dietary tryptophan to serotonin, resulting in pellagra.
- Recognize anxiety related to test results. Discuss the implications of abnormal test results on the patient’s lifestyle. Provide teaching and information regarding the clinical implications of the test results, as appropriate.
- Reinforce information given by the patient’s HCP regarding further testing, treatment, or referral to another HCP. Answer any questions or address any concerns voiced by the patient or family.
- Depending on the results of this procedure, additional testing may be performed to evaluate or monitor progression of the disease process and determine the need for a change in therapy. Evaluate test results in relation to the patient’s symptoms and other tests performed.
Related Monographs - Related tests include ALP, amino acid screen, antibodies gliadin, biopsy intestine, biopsy lung, calcium, cancer antigens, capsule endoscopy, chloride sweat, fecal fat, folate.
- Refer to the Endocrine, Gastrointestinal, and Immune systems tables at the end of the book for related tests by body system.
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