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Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines.
Biochem Biophys Res Commun. 1999 Feb 16; 255(2):470-6.BB

Abstract

The eosinophil chemotactic beta-chemokine MCP-4 is assumed to be involved in the accumulation of eosinophils characteristic for eosinophilic inflammatory diseases. We here describe the genomic organisation (3 exons of 138, 115 and 578 bp, 2 introns of 867 and 437 bp and 1.4 kb of regulatory sequences from the immediate 5' upstream region), sequence (genomic and transcribed) and mRNA expression of the human MCP-4 gene in dermal fibroblasts. Among the promoter elements potentially regulating MCP-4 gene expression and/or mediating the effects of anti-inflammatory drugs we identified consensus sequences known to interact with nuclear factors like NF-IL6, AP-2, a NF-kappaB like consensus sequence, gamma-interferon- response and YY-1 elements as well as glucocorticoid response elements. Like MCP-3, MCP-4 mRNA expression in dermal fibroblasts is upregulated by TNF-alpha, IL-1alpha, IFN-gamma or IL-4 and differs from RANTES and eotaxin mRNA expression in its response to IFN-gamma and/or IL-4.

Authors+Show Affiliations

Clinical Research Unit, Department of Dermatology, University of Kiel, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10049733

Citation

Hein, H, et al. "Genomic Organization, Sequence Analysis and Transcriptional Regulation of the Human MCP-4 Chemokine Gene (SCYA13) in Dermal Fibroblasts: a Comparison to Other Eosinophilic Beta-chemokines." Biochemical and Biophysical Research Communications, vol. 255, no. 2, 1999, pp. 470-6.
Hein H, Schlüter C, Kulke R, et al. Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines. Biochem Biophys Res Commun. 1999;255(2):470-6.
Hein, H., Schlüter, C., Kulke, R., Christophers, E., Schröder, J. M., & Bartels, J. (1999). Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines. Biochemical and Biophysical Research Communications, 255(2), 470-6.
Hein H, et al. Genomic Organization, Sequence Analysis and Transcriptional Regulation of the Human MCP-4 Chemokine Gene (SCYA13) in Dermal Fibroblasts: a Comparison to Other Eosinophilic Beta-chemokines. Biochem Biophys Res Commun. 1999 Feb 16;255(2):470-6. PubMed PMID: 10049733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines. AU - Hein,H, AU - Schlüter,C, AU - Kulke,R, AU - Christophers,E, AU - Schröder,J M, AU - Bartels,J, PY - 1999/3/2/pubmed PY - 1999/3/2/medline PY - 1999/3/2/entrez SP - 470 EP - 6 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 255 IS - 2 N2 - The eosinophil chemotactic beta-chemokine MCP-4 is assumed to be involved in the accumulation of eosinophils characteristic for eosinophilic inflammatory diseases. We here describe the genomic organisation (3 exons of 138, 115 and 578 bp, 2 introns of 867 and 437 bp and 1.4 kb of regulatory sequences from the immediate 5' upstream region), sequence (genomic and transcribed) and mRNA expression of the human MCP-4 gene in dermal fibroblasts. Among the promoter elements potentially regulating MCP-4 gene expression and/or mediating the effects of anti-inflammatory drugs we identified consensus sequences known to interact with nuclear factors like NF-IL6, AP-2, a NF-kappaB like consensus sequence, gamma-interferon- response and YY-1 elements as well as glucocorticoid response elements. Like MCP-3, MCP-4 mRNA expression in dermal fibroblasts is upregulated by TNF-alpha, IL-1alpha, IFN-gamma or IL-4 and differs from RANTES and eotaxin mRNA expression in its response to IFN-gamma and/or IL-4. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/10049733/Genomic_organization_sequence_analysis_and_transcriptional_regulation_of_the_human_MCP_4_chemokine_gene__SCYA13__in_dermal_fibroblasts:_a_comparison_to_other_eosinophilic_beta_chemokines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(99)90216-4 DB - PRIME DP - Unbound Medicine ER -