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Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity.
Proc Natl Acad Sci U S A. 1999 Mar 02; 96(5):2543-8.PN

Abstract

This study was undertaken to determine the modulation of uterine function by chorionic gonadotrophin (CG) in a nonhuman primate. Infusion of recombinant human CG (hCG) between days 6 and 10 post ovulation initiated the endoreplication of the uterine surface epithelium to form distinct epithelial plaques. These plaque cells stained intensely for cytokeratin and the proliferating cell nuclear antigen. The stromal fibroblasts below the epithelial plaques stained positively for alpha-smooth muscle actin (alphaSMA). Expression of alphaSMA is associated with the initiation of decidualization in the baboon endometrium. Synthesis of the glandular secretory protein glycodelin, as assessed by Western blot analysis, was markedly up-regulated by hCG, and this increase was confirmed by immunocytochemistry, Northern blot analysis, and reverse transcriptase-PCR. To determine whether hCG directly modulated these uterine responses, we treated ovariectomized baboons sequentially with estradiol and progesterone to mimic the hormonal profile of the normal menstrual cycle. Infusion of hCG into the oviduct of steroid-hormone-treated ovariectomized baboons induced the expression of alphaSMA in the stromal cells and glycodelin in the glandular epithelium. The epithelial plaque reaction, however, was not readily evident. These studies demonstrate a physiological effect of CG on the uterine endometrium in vivo and suggest that the primate blastocyst signal, like the blastocyst signals of other species, modulates the uterine environment prior to implantation.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University of Illinois, Chicago, IL 60612, USA. asgi@uic.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10051679

Citation

Fazleabas, A T., et al. "Modulation of the Baboon (Papio Anubis) Uterine Endometrium By Chorionic Gonadotrophin During the Period of Uterine Receptivity." Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 5, 1999, pp. 2543-8.
Fazleabas AT, Donnelly KM, Srinivasan S, et al. Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity. Proc Natl Acad Sci USA. 1999;96(5):2543-8.
Fazleabas, A. T., Donnelly, K. M., Srinivasan, S., Fortman, J. D., & Miller, J. B. (1999). Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity. Proceedings of the National Academy of Sciences of the United States of America, 96(5), 2543-8.
Fazleabas AT, et al. Modulation of the Baboon (Papio Anubis) Uterine Endometrium By Chorionic Gonadotrophin During the Period of Uterine Receptivity. Proc Natl Acad Sci USA. 1999 Mar 2;96(5):2543-8. PubMed PMID: 10051679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity. AU - Fazleabas,A T, AU - Donnelly,K M, AU - Srinivasan,S, AU - Fortman,J D, AU - Miller,J B, PY - 1999/3/3/pubmed PY - 1999/3/3/medline PY - 1999/3/3/entrez SP - 2543 EP - 8 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 96 IS - 5 N2 - This study was undertaken to determine the modulation of uterine function by chorionic gonadotrophin (CG) in a nonhuman primate. Infusion of recombinant human CG (hCG) between days 6 and 10 post ovulation initiated the endoreplication of the uterine surface epithelium to form distinct epithelial plaques. These plaque cells stained intensely for cytokeratin and the proliferating cell nuclear antigen. The stromal fibroblasts below the epithelial plaques stained positively for alpha-smooth muscle actin (alphaSMA). Expression of alphaSMA is associated with the initiation of decidualization in the baboon endometrium. Synthesis of the glandular secretory protein glycodelin, as assessed by Western blot analysis, was markedly up-regulated by hCG, and this increase was confirmed by immunocytochemistry, Northern blot analysis, and reverse transcriptase-PCR. To determine whether hCG directly modulated these uterine responses, we treated ovariectomized baboons sequentially with estradiol and progesterone to mimic the hormonal profile of the normal menstrual cycle. Infusion of hCG into the oviduct of steroid-hormone-treated ovariectomized baboons induced the expression of alphaSMA in the stromal cells and glycodelin in the glandular epithelium. The epithelial plaque reaction, however, was not readily evident. These studies demonstrate a physiological effect of CG on the uterine endometrium in vivo and suggest that the primate blastocyst signal, like the blastocyst signals of other species, modulates the uterine environment prior to implantation. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/10051679/Modulation_of_the_baboon__Papio_anubis__uterine_endometrium_by_chorionic_gonadotrophin_during_the_period_of_uterine_receptivity_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=10051679 DB - PRIME DP - Unbound Medicine ER -