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Selective trace enrichment by immunoaffinity capillary electrochromatography on-line with capillary zone electrophoresis - laser-induced fluorescence.
Electrophoresis. 1999 Jan; 20(1):57-66.E

Abstract

Limited by the lack of a sensitive, universal detector, many capillary-based liquid-phase separation techniques might benefit from techniques that overcome modest concentration sensitivity by preconcentrating large injection volumes. The work presented employs selective solid-phase extraction by immunoaffinity capillary electrochromatography (IACEC) to enhance detection limits. A model analyte, fluorescein isothiocyanate (FITC) biotin, is electrokinetically applied to a capillary column packed with an immobilized anti-biotin-IgG support. After selective extraction by the immunoaffinity capillary, the bound analyte is eluted, migrates by capillary zone electrophoresis (CZE), and is detected by laser-induced fluorescence. The column is regenerated and reused many times. We evaluate the performance of IACEC for selective trace enrichment of analytes prior to CZE. The calibration curve for FITC-biotin bound versus application time is linear from 10 to 300 seconds. Recovery of FITC-biotin spiked into a diluted urinary metabolites solution was 89.4% versus spiked buffer, with a precision of 1.8% relative standard deviation (RSD).

Authors+Show Affiliations

Sandia National Laboratories, Livermore, CA 94551, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10065959

Citation

Thomas, D H., et al. "Selective Trace Enrichment By Immunoaffinity Capillary Electrochromatography On-line With Capillary Zone Electrophoresis - Laser-induced Fluorescence." Electrophoresis, vol. 20, no. 1, 1999, pp. 57-66.
Thomas DH, Rakestraw DJ, Schoeniger JS, et al. Selective trace enrichment by immunoaffinity capillary electrochromatography on-line with capillary zone electrophoresis - laser-induced fluorescence. Electrophoresis. 1999;20(1):57-66.
Thomas, D. H., Rakestraw, D. J., Schoeniger, J. S., Lopez-Avila, V., & Van Emon, J. (1999). Selective trace enrichment by immunoaffinity capillary electrochromatography on-line with capillary zone electrophoresis - laser-induced fluorescence. Electrophoresis, 20(1), 57-66.
Thomas DH, et al. Selective Trace Enrichment By Immunoaffinity Capillary Electrochromatography On-line With Capillary Zone Electrophoresis - Laser-induced Fluorescence. Electrophoresis. 1999;20(1):57-66. PubMed PMID: 10065959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective trace enrichment by immunoaffinity capillary electrochromatography on-line with capillary zone electrophoresis - laser-induced fluorescence. AU - Thomas,D H, AU - Rakestraw,D J, AU - Schoeniger,J S, AU - Lopez-Avila,V, AU - Van Emon,J, PY - 1999/3/5/pubmed PY - 2000/8/12/medline PY - 1999/3/5/entrez SP - 57 EP - 66 JF - Electrophoresis JO - Electrophoresis VL - 20 IS - 1 N2 - Limited by the lack of a sensitive, universal detector, many capillary-based liquid-phase separation techniques might benefit from techniques that overcome modest concentration sensitivity by preconcentrating large injection volumes. The work presented employs selective solid-phase extraction by immunoaffinity capillary electrochromatography (IACEC) to enhance detection limits. A model analyte, fluorescein isothiocyanate (FITC) biotin, is electrokinetically applied to a capillary column packed with an immobilized anti-biotin-IgG support. After selective extraction by the immunoaffinity capillary, the bound analyte is eluted, migrates by capillary zone electrophoresis (CZE), and is detected by laser-induced fluorescence. The column is regenerated and reused many times. We evaluate the performance of IACEC for selective trace enrichment of analytes prior to CZE. The calibration curve for FITC-biotin bound versus application time is linear from 10 to 300 seconds. Recovery of FITC-biotin spiked into a diluted urinary metabolites solution was 89.4% versus spiked buffer, with a precision of 1.8% relative standard deviation (RSD). SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10065959/Selective_trace_enrichment_by_immunoaffinity_capillary_electrochromatography_on_line_with_capillary_zone_electrophoresis___laser_induced_fluorescence_ L2 - https://doi.org/10.1002/(SICI)1522-2683(19990101)20:1<57::AID-ELPS57>3.0.CO;2-J DB - PRIME DP - Unbound Medicine ER -