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Separation of linoleic acid oxidation products by micellar electrokinetic capillary chromatography and nonaqueous capillary electrophoresis.
Electrophoresis. 1999 Jan; 20(1):111-7.E

Abstract

In this work the suitability of micellar electrokinetic capillary chromatography (MEKC) and nonaqueous capillary electrophoresis (CE) to the analysis of the primary oxidation products of linoleic acid was studied with uncoated fused-silica capillaries. The primary autoxidation products of linoleic acid are the four hydroperoxide isomers 13-hydroperoxy-cis-9, trans-11-octadecadienoic acid, 13-hydroperoxy-trans-9, trans-11-octadecadienoic acid, 9-hydroperoxy-trans-10,cis-12-octadecadienoic acid, 9-hydroperoxy-trans-10, trans-12-octadecadienoic acid. Addition of a surfactant such as sodium dodecyl sulfate (SDS) or sodium cholate (SC) into the running buffer (20-30 mM 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) or ammonium acetate, pH 9.5-11) was required to enhance the water solubility of the sample and selectivity of the separation. MEKC proved to be a promising new technique for the separation of the primary oxidation products of lipids giving results comparable to high performance liquid chromatography (HPLC). Partial separation of hydroperoxide isomers was also achieved using nonaqueous CE with methanol-acetonitrile-sodium cholate as running buffer.

Authors+Show Affiliations

Jussila M
Department of Chemistry, University of Helsinki, Finland.
Sundberg S
No affiliation info available
Hopia A
No affiliation info available
Mäkinen M
No affiliation info available
Riekkola ML
No affiliation info available

MeSH

Chromatography, Micellar Electrokinetic CapillaryElectrophoresis, CapillaryLinoleic AcidOxidation-Reduction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10065966

Citation

Jussila, M, et al. "Separation of Linoleic Acid Oxidation Products By Micellar Electrokinetic Capillary Chromatography and Nonaqueous Capillary Electrophoresis." Electrophoresis, vol. 20, no. 1, 1999, pp. 111-7.
Jussila M, Sundberg S, Hopia A, et al. Separation of linoleic acid oxidation products by micellar electrokinetic capillary chromatography and nonaqueous capillary electrophoresis. Electrophoresis. 1999;20(1):111-7.
Jussila, M., Sundberg, S., Hopia, A., Mäkinen, M., & Riekkola, M. L. (1999). Separation of linoleic acid oxidation products by micellar electrokinetic capillary chromatography and nonaqueous capillary electrophoresis. Electrophoresis, 20(1), 111-7.
Jussila M, et al. Separation of Linoleic Acid Oxidation Products By Micellar Electrokinetic Capillary Chromatography and Nonaqueous Capillary Electrophoresis. Electrophoresis. 1999;20(1):111-7. PubMed PMID: 10065966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of linoleic acid oxidation products by micellar electrokinetic capillary chromatography and nonaqueous capillary electrophoresis. AU - Jussila,M, AU - Sundberg,S, AU - Hopia,A, AU - Mäkinen,M, AU - Riekkola,M L, PY - 1999/3/5/pubmed PY - 2000/8/12/medline PY - 1999/3/5/entrez SP - 111 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 20 IS - 1 N2 - In this work the suitability of micellar electrokinetic capillary chromatography (MEKC) and nonaqueous capillary electrophoresis (CE) to the analysis of the primary oxidation products of linoleic acid was studied with uncoated fused-silica capillaries. The primary autoxidation products of linoleic acid are the four hydroperoxide isomers 13-hydroperoxy-cis-9, trans-11-octadecadienoic acid, 13-hydroperoxy-trans-9, trans-11-octadecadienoic acid, 9-hydroperoxy-trans-10,cis-12-octadecadienoic acid, 9-hydroperoxy-trans-10, trans-12-octadecadienoic acid. Addition of a surfactant such as sodium dodecyl sulfate (SDS) or sodium cholate (SC) into the running buffer (20-30 mM 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) or ammonium acetate, pH 9.5-11) was required to enhance the water solubility of the sample and selectivity of the separation. MEKC proved to be a promising new technique for the separation of the primary oxidation products of lipids giving results comparable to high performance liquid chromatography (HPLC). Partial separation of hydroperoxide isomers was also achieved using nonaqueous CE with methanol-acetonitrile-sodium cholate as running buffer. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10065966/Separation_of_linoleic_acid_oxidation_products_by_micellar_electrokinetic_capillary_chromatography_and_nonaqueous_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -