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Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography.
Electrophoresis. 1999 Jan; 20(1):118-26.E

Abstract

Fast, efficient separation of most common biogenic amines was successfully performed on a glass microchip capillary electrophoresis device. The amines putrescine, histamine, tyramine, cadaverine, phenethylamine, tryptamine, spermidine and spermine were derivatized prior to fluorescence detection with fluorescein isothiocyanate. Separation was carried out using a channel length of 28 mm, a cross section of 50 x 8 microm, and a field strength of 600 V/cm. After optimization of buffer electrolyte conditions (120 mM boric acid, pH 9.4, modified with 40 mM SDS), fluorescein thiocarbamyl amine derivatives were successfully resolved. Analysis time was as short as 75 s. Determination of the biogenic amines was achieved in soy sauce samples.

Authors+Show Affiliations

Rodríguez I
Department of Chemistry, National University of Singapore, Republic of Singapore.
Lee HK
No affiliation info available
Li SF
No affiliation info available

MeSH

Biogenic AminesChromatography, Micellar Electrokinetic CapillaryFluorescein-5-isothiocyanateFluorescent Dyes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10065967

Citation

Rodríguez, I, et al. "Microchannel Electrophoretic Separation of Biogenic Amines By Micellar Electrokinetic Chromatography." Electrophoresis, vol. 20, no. 1, 1999, pp. 118-26.
Rodríguez I, Lee HK, Li SF. Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography. Electrophoresis. 1999;20(1):118-26.
Rodríguez, I., Lee, H. K., & Li, S. F. (1999). Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography. Electrophoresis, 20(1), 118-26.
Rodríguez I, Lee HK, Li SF. Microchannel Electrophoretic Separation of Biogenic Amines By Micellar Electrokinetic Chromatography. Electrophoresis. 1999;20(1):118-26. PubMed PMID: 10065967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography. AU - Rodríguez,I, AU - Lee,H K, AU - Li,S F, PY - 1999/3/5/pubmed PY - 2000/8/12/medline PY - 1999/3/5/entrez SP - 118 EP - 26 JF - Electrophoresis JO - Electrophoresis VL - 20 IS - 1 N2 - Fast, efficient separation of most common biogenic amines was successfully performed on a glass microchip capillary electrophoresis device. The amines putrescine, histamine, tyramine, cadaverine, phenethylamine, tryptamine, spermidine and spermine were derivatized prior to fluorescence detection with fluorescein isothiocyanate. Separation was carried out using a channel length of 28 mm, a cross section of 50 x 8 microm, and a field strength of 600 V/cm. After optimization of buffer electrolyte conditions (120 mM boric acid, pH 9.4, modified with 40 mM SDS), fluorescein thiocarbamyl amine derivatives were successfully resolved. Analysis time was as short as 75 s. Determination of the biogenic amines was achieved in soy sauce samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10065967/Microchannel_electrophoretic_separation_of_biogenic_amines_by_micellar_electrokinetic_chromatography_ L2 - https://doi.org/10.1002/(SICI)1522-2683(19990101)20:1<118::AID-ELPS118>3.0.CO;2-2 DB - PRIME DP - Unbound Medicine ER -
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