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Micellar electrokinetic capillary chromatography for the separation of cefalexin and its related substances.
Electrophoresis. 1999 Jan; 20(1):127-31.E

Abstract

Micellar electrokinetic capillary chromatography (MEKC) was examined for analysis of cefalexin and its related substances. Good selectivity was obtained with two different buffer solutions: a sodium acetate buffer (50 mM, pH 5.25) containing sodium dodecyl sulfate (50 mM SDS) or sodium phosphate buffer (40 mM, pH 7.0) containing 100 mM SDS. Both methods permit cefalexin to be completely separated from its ten related substances within 20 min. The robustness of the method, using pH 5.25 acetate buffer, was examined by means of a full-fraction factorial design to test the influence of buffer pH, concentration of SDS and buffer concentration. The parameters for validation such as linearity, precision, limit of detection and limit of quantitation are also reported. The results show that method 1 is suitable for the analysis of cefalexin.

Authors+Show Affiliations

Li YM
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, K. U. Leuven, Belgium.
Zhu Y
No affiliation info available
Vanderghinste D
No affiliation info available
Van Schepdael A
No affiliation info available
Roets E
No affiliation info available
Hoogmartens J
No affiliation info available

MeSH

CephalexinChromatography, Micellar Electrokinetic CapillaryMolecular Structure

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10065968

Citation

Li, Y M., et al. "Micellar Electrokinetic Capillary Chromatography for the Separation of Cefalexin and Its Related Substances." Electrophoresis, vol. 20, no. 1, 1999, pp. 127-31.
Li YM, Zhu Y, Vanderghinste D, et al. Micellar electrokinetic capillary chromatography for the separation of cefalexin and its related substances. Electrophoresis. 1999;20(1):127-31.
Li, Y. M., Zhu, Y., Vanderghinste, D., Van Schepdael, A., Roets, E., & Hoogmartens, J. (1999). Micellar electrokinetic capillary chromatography for the separation of cefalexin and its related substances. Electrophoresis, 20(1), 127-31.
Li YM, et al. Micellar Electrokinetic Capillary Chromatography for the Separation of Cefalexin and Its Related Substances. Electrophoresis. 1999;20(1):127-31. PubMed PMID: 10065968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar electrokinetic capillary chromatography for the separation of cefalexin and its related substances. AU - Li,Y M, AU - Zhu,Y, AU - Vanderghinste,D, AU - Van Schepdael,A, AU - Roets,E, AU - Hoogmartens,J, PY - 1999/3/5/pubmed PY - 2000/8/12/medline PY - 1999/3/5/entrez SP - 127 EP - 31 JF - Electrophoresis JO - Electrophoresis VL - 20 IS - 1 N2 - Micellar electrokinetic capillary chromatography (MEKC) was examined for analysis of cefalexin and its related substances. Good selectivity was obtained with two different buffer solutions: a sodium acetate buffer (50 mM, pH 5.25) containing sodium dodecyl sulfate (50 mM SDS) or sodium phosphate buffer (40 mM, pH 7.0) containing 100 mM SDS. Both methods permit cefalexin to be completely separated from its ten related substances within 20 min. The robustness of the method, using pH 5.25 acetate buffer, was examined by means of a full-fraction factorial design to test the influence of buffer pH, concentration of SDS and buffer concentration. The parameters for validation such as linearity, precision, limit of detection and limit of quantitation are also reported. The results show that method 1 is suitable for the analysis of cefalexin. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10065968/Micellar_electrokinetic_capillary_chromatography_for_the_separation_of_cefalexin_and_its_related_substances_ L2 - https://doi.org/10.1002/(SICI)1522-2683(19990101)20:1<127::AID-ELPS127>3.0.CO;2-2 DB - PRIME DP - Unbound Medicine ER -