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Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography.
Electrophoresis. 1999 Jan; 20(1):132-7.E

Abstract

A capillary electrophoresis (CE) method has been developed for simple and direct separation of cis- and trans-12,13-epoxy-9(Z)-octadecenoic acid and 9,10-epoxy-12(Z)-octadecenoic acid isomers. Separation was performed in micellar electrokinetic capillary chromatography (MEKC) using a buffer consisting of 25 mM borate (pH 9.20), 10 mM sodium dodecyl sulfate (SDS) and 10% v/v acetonitrile. The key variables, concentrations of SDS and organic modifier, were optimized by the application of a factorial experimental design. The use of a low micellar concentration, just above critical micelle concentration (CMC), in a background electrolyte containing an organic modifier not only made it possible to dissolve and separate highly hydrophobic fatty acid isomers, but also resulted in improved separation efficiency and selectivity. Separation efficiency up to 4 x 10(5) theoretical plates/m was achieved under an optimized condition. Also investigated were the influence of temperature on separation and the effect of organic modifier concentration on the dynamic exchange of the analytes between micelles and the bulk of the buffer solution. Direct UV was applied for detection of the fatty acids.

Authors+Show Affiliations

Wan H
Department of Chemistry, Karlstad University, Sweden.
Blomberg LG
No affiliation info available
Hamberg M
No affiliation info available

MeSH

Chromatography, Micellar Electrokinetic CapillaryElectrophoresis, CapillaryFatty AcidsIsomerismLinoleic AcidsMolecular StructureOleic AcidsReproducibility of ResultsResearch DesignTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10065969

Citation

Wan, H, et al. "Highly Efficient Separation of Isomeric Epoxy Fatty Acids By Micellar Electrokinetic Chromatography." Electrophoresis, vol. 20, no. 1, 1999, pp. 132-7.
Wan H, Blomberg LG, Hamberg M. Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography. Electrophoresis. 1999;20(1):132-7.
Wan, H., Blomberg, L. G., & Hamberg, M. (1999). Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography. Electrophoresis, 20(1), 132-7.
Wan H, Blomberg LG, Hamberg M. Highly Efficient Separation of Isomeric Epoxy Fatty Acids By Micellar Electrokinetic Chromatography. Electrophoresis. 1999;20(1):132-7. PubMed PMID: 10065969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography. AU - Wan,H, AU - Blomberg,L G, AU - Hamberg,M, PY - 1999/3/5/pubmed PY - 2000/8/12/medline PY - 1999/3/5/entrez SP - 132 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 20 IS - 1 N2 - A capillary electrophoresis (CE) method has been developed for simple and direct separation of cis- and trans-12,13-epoxy-9(Z)-octadecenoic acid and 9,10-epoxy-12(Z)-octadecenoic acid isomers. Separation was performed in micellar electrokinetic capillary chromatography (MEKC) using a buffer consisting of 25 mM borate (pH 9.20), 10 mM sodium dodecyl sulfate (SDS) and 10% v/v acetonitrile. The key variables, concentrations of SDS and organic modifier, were optimized by the application of a factorial experimental design. The use of a low micellar concentration, just above critical micelle concentration (CMC), in a background electrolyte containing an organic modifier not only made it possible to dissolve and separate highly hydrophobic fatty acid isomers, but also resulted in improved separation efficiency and selectivity. Separation efficiency up to 4 x 10(5) theoretical plates/m was achieved under an optimized condition. Also investigated were the influence of temperature on separation and the effect of organic modifier concentration on the dynamic exchange of the analytes between micelles and the bulk of the buffer solution. Direct UV was applied for detection of the fatty acids. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10065969/Highly_efficient_separation_of_isomeric_epoxy_fatty_acids_by_micellar_electrokinetic_chromatography_ L2 - https://doi.org/10.1002/(SICI)1522-2683(19990101)20:1<132::AID-ELPS132>3.0.CO;2-I DB - PRIME DP - Unbound Medicine ER -