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Fungal extracellular polysaccharides in house dust as a marker for exposure to fungi: relations with culturable fungi, reported home dampness, and respiratory symptoms.
J Allergy Clin Immunol. 1999 Mar; 103(3 Pt 1):494-500.JA

Abstract

BACKGROUND

Epidemiologic studies have demonstrated an association between indoor fungal growth and respiratory symptoms. However, in only a few studies was fungal exposure actually measured.

OBJECTIVE

The purpose of this study was to evaluate the measurement by enzyme immunoassay of extracellular polysaccharides of Aspergillus and Penicillium species (EPS-Asp/Pen) in house dust as a marker for fungal exposure and to study the relations between EPS-Asp/Pen levels and home dampness and respiratory symptoms in children.

METHODS

Extracts of house dust samples from bedroom and living room floors and mattresses from homes of 31 children with chronic respiratory symptoms and 29 children with no chronic respiratory symptoms were analyzed for EPS-Asp/Pen.

RESULTS

EPS-Asp/Pen were readily detectable (40 to 46,513 nanogram equivalent/g dust) in 161 house dust extracts, with highest concentrations in living room floor dust. EPS-Asp/Pen levels were 2 to 3 times higher on carpeted floors than on smooth floors. EPS-Asp/Pen were significantly correlated with total culturable fungi (r = 0.3 to 0.5) and with house dust mite allergens (r = 0.3 to 0.5). EPS-Asp/Pen levels in living room floor dust were positively associated with occupant-reported home dampness. This was not observed for EPS-Asp/Pen in bedroom floor and mattress dust. EPS-Asp/Pen levels in living room floor dust were positively associated with respiratory symptoms. EPS-Asp/Pen in bedroom floor and mattress dust showed a reversed association with respiratory symptoms, possibly because of allergen-avoidance measures taken in the bedroom.

CONCLUSION

The enzyme immunoassay for fungal EPS-Asp/Pen may be a useful method for exposure assessment of indoor fungi.

Authors+Show Affiliations

Department of Environmental Sciences, Environmental and Occupational Health Group, Wageningen Agricultural University, Wageningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10069885

Citation

Douwes, J, et al. "Fungal Extracellular Polysaccharides in House Dust as a Marker for Exposure to Fungi: Relations With Culturable Fungi, Reported Home Dampness, and Respiratory Symptoms." The Journal of Allergy and Clinical Immunology, vol. 103, no. 3 Pt 1, 1999, pp. 494-500.
Douwes J, van der Sluis B, Doekes G, et al. Fungal extracellular polysaccharides in house dust as a marker for exposure to fungi: relations with culturable fungi, reported home dampness, and respiratory symptoms. J Allergy Clin Immunol. 1999;103(3 Pt 1):494-500.
Douwes, J., van der Sluis, B., Doekes, G., van Leusden, F., Wijnands, L., van Strien, R., Verhoeff, A., & Brunekreef, B. (1999). Fungal extracellular polysaccharides in house dust as a marker for exposure to fungi: relations with culturable fungi, reported home dampness, and respiratory symptoms. The Journal of Allergy and Clinical Immunology, 103(3 Pt 1), 494-500.
Douwes J, et al. Fungal Extracellular Polysaccharides in House Dust as a Marker for Exposure to Fungi: Relations With Culturable Fungi, Reported Home Dampness, and Respiratory Symptoms. J Allergy Clin Immunol. 1999;103(3 Pt 1):494-500. PubMed PMID: 10069885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fungal extracellular polysaccharides in house dust as a marker for exposure to fungi: relations with culturable fungi, reported home dampness, and respiratory symptoms. AU - Douwes,J, AU - van der Sluis,B, AU - Doekes,G, AU - van Leusden,F, AU - Wijnands,L, AU - van Strien,R, AU - Verhoeff,A, AU - Brunekreef,B, PY - 1999/3/9/pubmed PY - 1999/3/9/medline PY - 1999/3/9/entrez SP - 494 EP - 500 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 103 IS - 3 Pt 1 N2 - BACKGROUND: Epidemiologic studies have demonstrated an association between indoor fungal growth and respiratory symptoms. However, in only a few studies was fungal exposure actually measured. OBJECTIVE: The purpose of this study was to evaluate the measurement by enzyme immunoassay of extracellular polysaccharides of Aspergillus and Penicillium species (EPS-Asp/Pen) in house dust as a marker for fungal exposure and to study the relations between EPS-Asp/Pen levels and home dampness and respiratory symptoms in children. METHODS: Extracts of house dust samples from bedroom and living room floors and mattresses from homes of 31 children with chronic respiratory symptoms and 29 children with no chronic respiratory symptoms were analyzed for EPS-Asp/Pen. RESULTS: EPS-Asp/Pen were readily detectable (40 to 46,513 nanogram equivalent/g dust) in 161 house dust extracts, with highest concentrations in living room floor dust. EPS-Asp/Pen levels were 2 to 3 times higher on carpeted floors than on smooth floors. EPS-Asp/Pen were significantly correlated with total culturable fungi (r = 0.3 to 0.5) and with house dust mite allergens (r = 0.3 to 0.5). EPS-Asp/Pen levels in living room floor dust were positively associated with occupant-reported home dampness. This was not observed for EPS-Asp/Pen in bedroom floor and mattress dust. EPS-Asp/Pen levels in living room floor dust were positively associated with respiratory symptoms. EPS-Asp/Pen in bedroom floor and mattress dust showed a reversed association with respiratory symptoms, possibly because of allergen-avoidance measures taken in the bedroom. CONCLUSION: The enzyme immunoassay for fungal EPS-Asp/Pen may be a useful method for exposure assessment of indoor fungi. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/10069885/Fungal_extracellular_polysaccharides_in_house_dust_as_a_marker_for_exposure_to_fungi:_relations_with_culturable_fungi_reported_home_dampness_and_respiratory_symptoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(99)70476-8 DB - PRIME DP - Unbound Medicine ER -