Tags

Type your tag names separated by a space and hit enter

[Risk of underestimating blood carbon monoxide by certain analytic methods].
Presse Med. 1999 Jan 30; 28(4):163-7.PM

Abstract

OBJECTIVE

Study the effect of delay to assay on the measurement of carboxyhemoglobin (HbCO) in total blood samples.

METHODS

Carbon monoxide (CO) and carboxyhemoglobin were measured on 75 blood samples drawn from healthy subjects (smokers and non smokers) and in subjects with carbon monoxide (CO) poisoning. Blood samples were drawn on lithium heparinate in perfectly closed tubes with no head space and stored at 4 infinity C until assay. The samples were pooled into 4 classes for 4 delays to assay: immediate, less than one hour, 3 hours, 12 hours. Infrared spectrometry was used to assay CO and order 4 and 5 derived spectrophotometry using CO-oximeters (AVL 912, IL 482, Corning 270, Radiometer OSM 3, Radiometer ABL 520) for HbCO.

RESULTS

Regression lines for CO versus HbCO suggested that oxycarbonemia was underestimated using techniques measuring HbCO. This underestimation varied from 3 to 40% for delays to assay of 0 to 3 hours.

CONCLUSION

Clinicians should be aware that the underestimation in oxycarbonemia related to HbCO assays is sensitive to delay to assay.

Authors+Show Affiliations

Gourlain H
Laboratoire de Biochime-Toxicologie, Hôpital Fernand Widal, Paris.
Laforge M
No affiliation info available
Buneaux F
No affiliation info available
Galliot-Guilley M
No affiliation info available

MeSH

AdultCarbon MonoxideCarbon Monoxide PoisoningCarboxyhemoglobinFemaleHumansMalePredictive Value of TestsSmokingSpectrophotometrySpectrophotometry, Infrared

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

fre

PubMed ID

10071626

Citation

Gourlain, H, et al. "[Risk of Underestimating Blood Carbon Monoxide By Certain Analytic Methods]." Presse Medicale (Paris, France : 1983), vol. 28, no. 4, 1999, pp. 163-7.
Gourlain H, Laforge M, Buneaux F, et al. [Risk of underestimating blood carbon monoxide by certain analytic methods]. Presse Med. 1999;28(4):163-7.
Gourlain, H., Laforge, M., Buneaux, F., & Galliot-Guilley, M. (1999). [Risk of underestimating blood carbon monoxide by certain analytic methods]. Presse Medicale (Paris, France : 1983), 28(4), 163-7.
Gourlain H, et al. [Risk of Underestimating Blood Carbon Monoxide By Certain Analytic Methods]. Presse Med. 1999 Jan 30;28(4):163-7. PubMed PMID: 10071626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Risk of underestimating blood carbon monoxide by certain analytic methods]. AU - Gourlain,H, AU - Laforge,M, AU - Buneaux,F, AU - Galliot-Guilley,M, PY - 1999/3/11/pubmed PY - 2000/2/26/medline PY - 1999/3/11/entrez SP - 163 EP - 7 JF - Presse medicale (Paris, France : 1983) JO - Presse Med VL - 28 IS - 4 N2 - OBJECTIVE: Study the effect of delay to assay on the measurement of carboxyhemoglobin (HbCO) in total blood samples. METHODS: Carbon monoxide (CO) and carboxyhemoglobin were measured on 75 blood samples drawn from healthy subjects (smokers and non smokers) and in subjects with carbon monoxide (CO) poisoning. Blood samples were drawn on lithium heparinate in perfectly closed tubes with no head space and stored at 4 infinity C until assay. The samples were pooled into 4 classes for 4 delays to assay: immediate, less than one hour, 3 hours, 12 hours. Infrared spectrometry was used to assay CO and order 4 and 5 derived spectrophotometry using CO-oximeters (AVL 912, IL 482, Corning 270, Radiometer OSM 3, Radiometer ABL 520) for HbCO. RESULTS: Regression lines for CO versus HbCO suggested that oxycarbonemia was underestimated using techniques measuring HbCO. This underestimation varied from 3 to 40% for delays to assay of 0 to 3 hours. CONCLUSION: Clinicians should be aware that the underestimation in oxycarbonemia related to HbCO assays is sensitive to delay to assay. SN - 0755-4982 UR - https://www.unboundmedicine.com/medline/citation/10071626/[Risk_of_underestimating_blood_carbon_monoxide_by_certain_analytic_methods]_ L2 - https://medlineplus.gov/carbonmonoxidepoisoning.html DB - PRIME DP - Unbound Medicine ER -