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A novel high-performance anion-exchange chromatographic method for the analysis of carrageenans and agars containing 3,6-anhydrogalactose.
Anal Biochem. 1999 Mar 15; 268(2):213-22.AB

Abstract

A novel method has been developed to determine the sugar composition of 3,6-anhydrogalactose-containing polysaccharides, such as carrageenan and agar. The method is based on reductive hydrolysis with a methylmorpholine-borane complex in the presence of acid and subsequent high-performance anion-exchange chromatography analysis of the alditols without any derivatization. The method was validated by 13C NMR analysis of six carrageenans and three agars and by a previously used method based on derivatization to alditol acetates and gas-liquid chromatography analysis. The new method was found to be superior to the gas-liquid chromatography method as the analysis time was less than half. Also it was found to be more accurate and reproducible and no derivatization was required. The analysis of the six different carrageenan samples revealed that homogeneous mu- and nu-carrageenan, theoretically without 3,6-anhydrogalactose residues, cannot be isolated from red seaweeds. Consequently, the question arose if mu- and nu-carrageenans at all are present in seaweeds and if the current hypotheses regarding biosynthesis of carrageenans in the seaweeds are correct. The data demonstrated that carrageenans are highly complex natural polysaccharides, which are more irregular than assumed hitherto. The new analytical technique will permit elucidation of the detailed structure of seaweed polysaccharides and determination of their structure-property relationships.

Authors+Show Affiliations

Hercules European Research Center, Barneveld, 3770 AG, The Netherlands.cjol@herc.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10075810

Citation

Jol, C N., et al. "A Novel High-performance Anion-exchange Chromatographic Method for the Analysis of Carrageenans and Agars Containing 3,6-anhydrogalactose." Analytical Biochemistry, vol. 268, no. 2, 1999, pp. 213-22.
Jol CN, Neiss TG, Penninkhof B, et al. A novel high-performance anion-exchange chromatographic method for the analysis of carrageenans and agars containing 3,6-anhydrogalactose. Anal Biochem. 1999;268(2):213-22.
Jol, C. N., Neiss, T. G., Penninkhof, B., Rudolph, B., & De Ruiter, G. A. (1999). A novel high-performance anion-exchange chromatographic method for the analysis of carrageenans and agars containing 3,6-anhydrogalactose. Analytical Biochemistry, 268(2), 213-22.
Jol CN, et al. A Novel High-performance Anion-exchange Chromatographic Method for the Analysis of Carrageenans and Agars Containing 3,6-anhydrogalactose. Anal Biochem. 1999 Mar 15;268(2):213-22. PubMed PMID: 10075810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel high-performance anion-exchange chromatographic method for the analysis of carrageenans and agars containing 3,6-anhydrogalactose. AU - Jol,C N, AU - Neiss,T G, AU - Penninkhof,B, AU - Rudolph,B, AU - De Ruiter,G A, PY - 1999/3/17/pubmed PY - 1999/3/17/medline PY - 1999/3/17/entrez SP - 213 EP - 22 JF - Analytical biochemistry JO - Anal Biochem VL - 268 IS - 2 N2 - A novel method has been developed to determine the sugar composition of 3,6-anhydrogalactose-containing polysaccharides, such as carrageenan and agar. The method is based on reductive hydrolysis with a methylmorpholine-borane complex in the presence of acid and subsequent high-performance anion-exchange chromatography analysis of the alditols without any derivatization. The method was validated by 13C NMR analysis of six carrageenans and three agars and by a previously used method based on derivatization to alditol acetates and gas-liquid chromatography analysis. The new method was found to be superior to the gas-liquid chromatography method as the analysis time was less than half. Also it was found to be more accurate and reproducible and no derivatization was required. The analysis of the six different carrageenan samples revealed that homogeneous mu- and nu-carrageenan, theoretically without 3,6-anhydrogalactose residues, cannot be isolated from red seaweeds. Consequently, the question arose if mu- and nu-carrageenans at all are present in seaweeds and if the current hypotheses regarding biosynthesis of carrageenans in the seaweeds are correct. The data demonstrated that carrageenans are highly complex natural polysaccharides, which are more irregular than assumed hitherto. The new analytical technique will permit elucidation of the detailed structure of seaweed polysaccharides and determination of their structure-property relationships. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/10075810/A_novel_high_performance_anion_exchange_chromatographic_method_for_the_analysis_of_carrageenans_and_agars_containing_36_anhydrogalactose_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(98)93059-7 DB - PRIME DP - Unbound Medicine ER -