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Cell type-specific ATP-activated responses in rat dorsal root ganglion neurons.
Br J Pharmacol 1999; 126(2):429-36BJ

Abstract

1. The aim of our study is to clarify the relationship between expression pattern of P2X receptors and the cell type of male adult rat (Wistar) dorsal root ganglion (DRG) neurons. We identified the nociceptive cells of acutely dissociated DRG neurons from adult rats type using capsaicin sensitivity. 2. Two types of ATP-activated currents, one with fast, the other with slow desensitization, were found under voltage-clamp conditions. In addition, cells with fast but not slow desensitization responded to capsaicin, indicating that there was a relationship between current kinetics and capsaicin-sensitivity. 3. Both types of neurons were responsive to ATP and alpha, beta methylene-ATP (alpha,betameATP). The concentration of alpha,(beta)meATP producing half-maximal activation (EC50) of neurons with fast desensitization was less (11 microM) than that of neurons with slow desensitization (63 microM), while the Hill coefficients were similar. Suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid tetrasodium (PPADS) antagonized alpha,betameATP-induced currents in both types of neurons. 4. In situ hybridization revealed that small cells of the DRG predominantly expressed mRNAs of P2X3 and medium-sized cells expressed mRNAs of P2X2 and P2X3. In contrast, both of mRNAs were not detected in large cells of the DRG. 5. These results suggest that capsaicin-sensitive, small-sized DRG neurons expressed mainly the homomeric P2X3 subunit and that capsaicin-insensitive, medium-sized DRG neurons expressed the heteromultimeric receptor with P2X2 and P2X3.

Authors+Show Affiliations

Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan. inoue@nihs.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10077235

Citation

Ueno, S, et al. "Cell Type-specific ATP-activated Responses in Rat Dorsal Root Ganglion Neurons." British Journal of Pharmacology, vol. 126, no. 2, 1999, pp. 429-36.
Ueno S, Tsuda M, Iwanaga T, et al. Cell type-specific ATP-activated responses in rat dorsal root ganglion neurons. Br J Pharmacol. 1999;126(2):429-36.
Ueno, S., Tsuda, M., Iwanaga, T., & Inoue, K. (1999). Cell type-specific ATP-activated responses in rat dorsal root ganglion neurons. British Journal of Pharmacology, 126(2), pp. 429-36.
Ueno S, et al. Cell Type-specific ATP-activated Responses in Rat Dorsal Root Ganglion Neurons. Br J Pharmacol. 1999;126(2):429-36. PubMed PMID: 10077235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell type-specific ATP-activated responses in rat dorsal root ganglion neurons. AU - Ueno,S, AU - Tsuda,M, AU - Iwanaga,T, AU - Inoue,K, PY - 1999/3/17/pubmed PY - 1999/3/17/medline PY - 1999/3/17/entrez SP - 429 EP - 36 JF - British journal of pharmacology JO - Br. J. Pharmacol. VL - 126 IS - 2 N2 - 1. The aim of our study is to clarify the relationship between expression pattern of P2X receptors and the cell type of male adult rat (Wistar) dorsal root ganglion (DRG) neurons. We identified the nociceptive cells of acutely dissociated DRG neurons from adult rats type using capsaicin sensitivity. 2. Two types of ATP-activated currents, one with fast, the other with slow desensitization, were found under voltage-clamp conditions. In addition, cells with fast but not slow desensitization responded to capsaicin, indicating that there was a relationship between current kinetics and capsaicin-sensitivity. 3. Both types of neurons were responsive to ATP and alpha, beta methylene-ATP (alpha,betameATP). The concentration of alpha,(beta)meATP producing half-maximal activation (EC50) of neurons with fast desensitization was less (11 microM) than that of neurons with slow desensitization (63 microM), while the Hill coefficients were similar. Suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid tetrasodium (PPADS) antagonized alpha,betameATP-induced currents in both types of neurons. 4. In situ hybridization revealed that small cells of the DRG predominantly expressed mRNAs of P2X3 and medium-sized cells expressed mRNAs of P2X2 and P2X3. In contrast, both of mRNAs were not detected in large cells of the DRG. 5. These results suggest that capsaicin-sensitive, small-sized DRG neurons expressed mainly the homomeric P2X3 subunit and that capsaicin-insensitive, medium-sized DRG neurons expressed the heteromultimeric receptor with P2X2 and P2X3. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/10077235/Cell_type_specific_ATP_activated_responses_in_rat_dorsal_root_ganglion_neurons_ L2 - https://doi.org/10.1038/sj.bjp.0702319 DB - PRIME DP - Unbound Medicine ER -