Tags

Type your tag names separated by a space and hit enter

Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+.
J Cell Physiol. 1999 Apr; 179(1):1-10.JC

Abstract

In order to better understand granule release from platelets, we developed an alpha-toxin permeabilized platelet model to study alpha-granule secretion. Secretion of alpha-granules was analyzed by flow cytometry using P-selectin as a marker for alpha-granule release. P-selectin surface expression occurred when platelets were permeabilized in the presence of Ca2+. Responsiveness to Ca2+ was lost 30 min after permeabilization but could be reconstituted with MgATP. Alpha-toxin-permeabilized, MgATP-exposed platelets also degranulated within a pH range of 5.4-5.9 without exposure to and independent of Ca2+. ATP, GTP, CTP, UTP, and ITP supported Ca2+-induced alpha-granule secretion, while H+-induced alpha-granule secretion occurred only with ATP and GTP. Both Ca2+- and H+-induced alpha-granule secretion required ATP hydrolysis. Kinase inhibitors blocked both Ca2+- and H+-induced secretion. These data suggest that alpha-granule secretion in this permeabilized platelet system shares many characteristics with granule secretion studied in other permeabilized cell models. Furthermore, these results show that H+ can trigger alpha-granule release independent of Ca2+.

Authors+Show Affiliations

Center for Thrombosis and Hemostasis Research, Boston, Massachusetts, USA. rflaumen@bidmc.harvard.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10082126

Citation

Flaumenhaft, R, et al. "Alpha-granule Secretion From Alpha-toxin Permeabilized, MgATP-exposed Platelets Is Induced Independently By H+ and Ca2+." Journal of Cellular Physiology, vol. 179, no. 1, 1999, pp. 1-10.
Flaumenhaft R, Furie B, Furie BC. Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+. J Cell Physiol. 1999;179(1):1-10.
Flaumenhaft, R., Furie, B., & Furie, B. C. (1999). Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+. Journal of Cellular Physiology, 179(1), 1-10.
Flaumenhaft R, Furie B, Furie BC. Alpha-granule Secretion From Alpha-toxin Permeabilized, MgATP-exposed Platelets Is Induced Independently By H+ and Ca2+. J Cell Physiol. 1999;179(1):1-10. PubMed PMID: 10082126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+. AU - Flaumenhaft,R, AU - Furie,B, AU - Furie,B C, PY - 1999/3/19/pubmed PY - 2000/6/20/medline PY - 1999/3/19/entrez SP - 1 EP - 10 JF - Journal of cellular physiology JO - J Cell Physiol VL - 179 IS - 1 N2 - In order to better understand granule release from platelets, we developed an alpha-toxin permeabilized platelet model to study alpha-granule secretion. Secretion of alpha-granules was analyzed by flow cytometry using P-selectin as a marker for alpha-granule release. P-selectin surface expression occurred when platelets were permeabilized in the presence of Ca2+. Responsiveness to Ca2+ was lost 30 min after permeabilization but could be reconstituted with MgATP. Alpha-toxin-permeabilized, MgATP-exposed platelets also degranulated within a pH range of 5.4-5.9 without exposure to and independent of Ca2+. ATP, GTP, CTP, UTP, and ITP supported Ca2+-induced alpha-granule secretion, while H+-induced alpha-granule secretion occurred only with ATP and GTP. Both Ca2+- and H+-induced alpha-granule secretion required ATP hydrolysis. Kinase inhibitors blocked both Ca2+- and H+-induced secretion. These data suggest that alpha-granule secretion in this permeabilized platelet system shares many characteristics with granule secretion studied in other permeabilized cell models. Furthermore, these results show that H+ can trigger alpha-granule release independent of Ca2+. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/10082126/Alpha_granule_secretion_from_alpha_toxin_permeabilized_MgATP_exposed_platelets_is_induced_independently_by_H+_and_Ca2+_ L2 - https://doi.org/10.1002/(SICI)1097-4652(199904)179:1<1::AID-JCP1>3.0.CO;2-E DB - PRIME DP - Unbound Medicine ER -