Tags

Type your tag names separated by a space and hit enter

Procyanidin oligomers selectively and intensively promote proliferation of mouse hair epithelial cells in vitro and activate hair follicle growth in vivo.
J Invest Dermatol. 1999 Mar; 112(3):310-6.JI

Abstract

We have previously reported that proanthocyanidins extracted from grape seeds possess growth-promoting activity toward murine hair epithelial cells in vitro and stimulate anagen induction in hair cycle progression in vivo. This report constitutes a comparison of the growth-promoting activity of procyanidin oligomers and the target cells of procyanidins in the skin. Results show that procyanidin dimer and trimer exhibit higher growth-promoting activity than the monomer. The maximum growth-promoting activity for hair epithelial cells with procyanidin B-2, an epicatechin dimer, reached about 300% (30 microM) relative to controls (= 100%) in a 5 d culture. Optimum concentration of procyanidin C-1, an epicatechin trimer, was lower than that of procyanidin B-2; the maximum growth-promoting activity of procyanidin C-1 was about 220% (3 microM). No other flavonoid compounds examined exhibit higher proliferative activities than the procyanidins. In skin constituent cells, only epithelial cells such as hair keratinocytes or epidermal keratinocytes respond to procyanidin oligomers. Topical application of 1% procyanidin oligomers on shaven C3H mice in the telogen phase led to significant hair regeneration [procyanidin B-2, 69.6% +/- 21.8% (mean +/- SD); procyanidin B-3, 80.9% +/- 13.0%; procyanidin C-1, 78.3% +/- 7.6%] on the basis of the shaven area; application of vehicle only led to regeneration of 41.7% (SD = 16.3%). In this paper, we demonstrate the hair-growing activity of procyanidin oligomers both in vitro and in vivo, and their potential for use as agents to induce hair growth.

Authors+Show Affiliations

Tsukuba Research Laboratories, Kyowa Hakko Kogyo, Ibaraki, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10084307

Citation

Takahashi, T, et al. "Procyanidin Oligomers Selectively and Intensively Promote Proliferation of Mouse Hair Epithelial Cells in Vitro and Activate Hair Follicle Growth in Vivo." The Journal of Investigative Dermatology, vol. 112, no. 3, 1999, pp. 310-6.
Takahashi T, Kamiya T, Hasegawa A, et al. Procyanidin oligomers selectively and intensively promote proliferation of mouse hair epithelial cells in vitro and activate hair follicle growth in vivo. J Invest Dermatol. 1999;112(3):310-6.
Takahashi, T., Kamiya, T., Hasegawa, A., & Yokoo, Y. (1999). Procyanidin oligomers selectively and intensively promote proliferation of mouse hair epithelial cells in vitro and activate hair follicle growth in vivo. The Journal of Investigative Dermatology, 112(3), 310-6.
Takahashi T, et al. Procyanidin Oligomers Selectively and Intensively Promote Proliferation of Mouse Hair Epithelial Cells in Vitro and Activate Hair Follicle Growth in Vivo. J Invest Dermatol. 1999;112(3):310-6. PubMed PMID: 10084307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Procyanidin oligomers selectively and intensively promote proliferation of mouse hair epithelial cells in vitro and activate hair follicle growth in vivo. AU - Takahashi,T, AU - Kamiya,T, AU - Hasegawa,A, AU - Yokoo,Y, PY - 1999/3/20/pubmed PY - 1999/3/20/medline PY - 1999/3/20/entrez SP - 310 EP - 6 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 112 IS - 3 N2 - We have previously reported that proanthocyanidins extracted from grape seeds possess growth-promoting activity toward murine hair epithelial cells in vitro and stimulate anagen induction in hair cycle progression in vivo. This report constitutes a comparison of the growth-promoting activity of procyanidin oligomers and the target cells of procyanidins in the skin. Results show that procyanidin dimer and trimer exhibit higher growth-promoting activity than the monomer. The maximum growth-promoting activity for hair epithelial cells with procyanidin B-2, an epicatechin dimer, reached about 300% (30 microM) relative to controls (= 100%) in a 5 d culture. Optimum concentration of procyanidin C-1, an epicatechin trimer, was lower than that of procyanidin B-2; the maximum growth-promoting activity of procyanidin C-1 was about 220% (3 microM). No other flavonoid compounds examined exhibit higher proliferative activities than the procyanidins. In skin constituent cells, only epithelial cells such as hair keratinocytes or epidermal keratinocytes respond to procyanidin oligomers. Topical application of 1% procyanidin oligomers on shaven C3H mice in the telogen phase led to significant hair regeneration [procyanidin B-2, 69.6% +/- 21.8% (mean +/- SD); procyanidin B-3, 80.9% +/- 13.0%; procyanidin C-1, 78.3% +/- 7.6%] on the basis of the shaven area; application of vehicle only led to regeneration of 41.7% (SD = 16.3%). In this paper, we demonstrate the hair-growing activity of procyanidin oligomers both in vitro and in vivo, and their potential for use as agents to induce hair growth. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/10084307/Procyanidin_oligomers_selectively_and_intensively_promote_proliferation_of_mouse_hair_epithelial_cells_in_vitro_and_activate_hair_follicle_growth_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)40418-X DB - PRIME DP - Unbound Medicine ER -