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Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12.
Plasmid 1999; 41(2):110-9P

Abstract

The copy number per cell mass of plasmid pBR322 and a rom- derivative was measured as a function of generation time. In fast growing cells the copy number per cell mass was virtually identical for rom+ and rom- derivatives. However, the copy number of pBR322 only increased 3- to 4-fold from a 20- to 80-min generation time, whereas the copy number of the rom- derivative increased 7- to 10-fold. The copy number stayed constant for the rom+ and rom- plasmids at generation times longer than 80-100 min. Thus, the presence of the rom gene decreased the copy number of plasmid pBR322 in slowly growing cells at least 2-fold when compared with the rom- plasmid. To study the effect of the rom gene in trans we cloned the gene into the compatible P15A-derived rom- plasmid pACYC184. In cells carrying both pACYC184 rom+ and pBR322 rom- the presence of the rom gene in trans had little effect on the copy number of pBR322 rom- at fast growth, but it decreased its copy number at slow growth to the same level as found for pBR322, i.e., complemented the pBR322 rom- plasmid. The pACYC184 plasmid and its rom+ derivatives showed copy numbers similar to those of pBR322 rom- and pBR322 itself, respectively, at fast and slow growth. We conclude that the rom gene product-the Rom protein-is an important element in copy number control of ColE1-type plasmids especially in slowly growing cells.

Authors+Show Affiliations

Department of Microbiology, Technical University of Denmark, Building 301, Lyngby, DK-2800, Denmark.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10087214

Citation

Atlung, T, et al. "Role of the Rom Protein in Copy Number Control of Plasmid pBR322 at Different Growth Rates in Escherichia Coli K-12." Plasmid, vol. 41, no. 2, 1999, pp. 110-9.
Atlung T, Christensen BB, Hansen FG. Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12. Plasmid. 1999;41(2):110-9.
Atlung, T., Christensen, B. B., & Hansen, F. G. (1999). Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12. Plasmid, 41(2), pp. 110-9.
Atlung T, Christensen BB, Hansen FG. Role of the Rom Protein in Copy Number Control of Plasmid pBR322 at Different Growth Rates in Escherichia Coli K-12. Plasmid. 1999;41(2):110-9. PubMed PMID: 10087214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12. AU - Atlung,T, AU - Christensen,B B, AU - Hansen,F G, PY - 1999/3/24/pubmed PY - 1999/3/24/medline PY - 1999/3/24/entrez SP - 110 EP - 9 JF - Plasmid JO - Plasmid VL - 41 IS - 2 N2 - The copy number per cell mass of plasmid pBR322 and a rom- derivative was measured as a function of generation time. In fast growing cells the copy number per cell mass was virtually identical for rom+ and rom- derivatives. However, the copy number of pBR322 only increased 3- to 4-fold from a 20- to 80-min generation time, whereas the copy number of the rom- derivative increased 7- to 10-fold. The copy number stayed constant for the rom+ and rom- plasmids at generation times longer than 80-100 min. Thus, the presence of the rom gene decreased the copy number of plasmid pBR322 in slowly growing cells at least 2-fold when compared with the rom- plasmid. To study the effect of the rom gene in trans we cloned the gene into the compatible P15A-derived rom- plasmid pACYC184. In cells carrying both pACYC184 rom+ and pBR322 rom- the presence of the rom gene in trans had little effect on the copy number of pBR322 rom- at fast growth, but it decreased its copy number at slow growth to the same level as found for pBR322, i.e., complemented the pBR322 rom- plasmid. The pACYC184 plasmid and its rom+ derivatives showed copy numbers similar to those of pBR322 rom- and pBR322 itself, respectively, at fast and slow growth. We conclude that the rom gene product-the Rom protein-is an important element in copy number control of ColE1-type plasmids especially in slowly growing cells. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/10087214/Role_of_the_rom_protein_in_copy_number_control_of_plasmid_pBR322_at_different_growth_rates_in_Escherichia_coli_K_12_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(98)91386-5 DB - PRIME DP - Unbound Medicine ER -