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Intraocular anti-Toxoplasma gondii IgA antibody production in patients with ocular toxoplasmosis.
Am J Ophthalmol 1999; 127(3):294-300AJ

Abstract

PURPOSE

To investigate the immunoglobulin classes associated with the intraocular anti-Toxoplasma gondii antibody response during clinical ocular toxoplasmosis and to determine which immunoglobulin class is most helpful in the diagnosis of this disease.

METHODS

Paired serum and intraocular fluid samples from 155 patients who had uveitis were tested for intraocular anti-T. gondii IgG, IgA, and IgM antibody production. The presence of T. gondii DNA was determined by polymerase chain reaction. Patients were divided into two groups, based on the initial clinical diagnosis; group 1 included 78 patients with presumed ocular toxoplasmosis, and group 2 included 77 patients with uveitis that was not clinically suspected to be ocular toxoplasmosis. Samples from 27 nonuveitis patients who underwent intraocular surgery were used as control subjects. The final diagnosis was based on the clinical course and interpretation of laboratory tests.

RESULTS

A final diagnosis of ocular toxoplasmosis was made in 88 of 155 patients (group 1, 68; group 2, 20). Among these patients, 65% had intraocular IgG production, 52% had intraocular IgA production, 37.5% had both IgG and IgA production, 27% had IgG production only, and 15% had IgA production only. Of the 13 patients tested, only one had intraocular IgM production. Intraocular IgA could not be detected in patients who had final diagnoses other than ocular toxoplasmosis or in control subjects. A positive polymerase chain reaction combined with a test that was positive for intraocular IgG production had a sensitivity of 77%, which increased to 91% after the detection of intraocular IgA production was added.

CONCLUSIONS

Immunoglobulin G is the major class involved in the humoral immune response against the T. gondii parasite, followed by IgA. The determination of IgA production is useful as an additional test in the diagnosis of ocular toxoplasmosis.

Authors+Show Affiliations

Netherlands Ophthalmic Research Institute, Amsterdam.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10088739

Citation

Ronday, M J., et al. "Intraocular anti-Toxoplasma Gondii IgA Antibody Production in Patients With Ocular Toxoplasmosis." American Journal of Ophthalmology, vol. 127, no. 3, 1999, pp. 294-300.
Ronday MJ, Ongkosuwito JV, Rothova A, et al. Intraocular anti-Toxoplasma gondii IgA antibody production in patients with ocular toxoplasmosis. Am J Ophthalmol. 1999;127(3):294-300.
Ronday, M. J., Ongkosuwito, J. V., Rothova, A., & Kijlstra, A. (1999). Intraocular anti-Toxoplasma gondii IgA antibody production in patients with ocular toxoplasmosis. American Journal of Ophthalmology, 127(3), pp. 294-300.
Ronday MJ, et al. Intraocular anti-Toxoplasma Gondii IgA Antibody Production in Patients With Ocular Toxoplasmosis. Am J Ophthalmol. 1999;127(3):294-300. PubMed PMID: 10088739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraocular anti-Toxoplasma gondii IgA antibody production in patients with ocular toxoplasmosis. AU - Ronday,M J, AU - Ongkosuwito,J V, AU - Rothova,A, AU - Kijlstra,A, PY - 1999/3/24/pubmed PY - 1999/3/24/medline PY - 1999/3/24/entrez SP - 294 EP - 300 JF - American journal of ophthalmology JO - Am. J. Ophthalmol. VL - 127 IS - 3 N2 - PURPOSE: To investigate the immunoglobulin classes associated with the intraocular anti-Toxoplasma gondii antibody response during clinical ocular toxoplasmosis and to determine which immunoglobulin class is most helpful in the diagnosis of this disease. METHODS: Paired serum and intraocular fluid samples from 155 patients who had uveitis were tested for intraocular anti-T. gondii IgG, IgA, and IgM antibody production. The presence of T. gondii DNA was determined by polymerase chain reaction. Patients were divided into two groups, based on the initial clinical diagnosis; group 1 included 78 patients with presumed ocular toxoplasmosis, and group 2 included 77 patients with uveitis that was not clinically suspected to be ocular toxoplasmosis. Samples from 27 nonuveitis patients who underwent intraocular surgery were used as control subjects. The final diagnosis was based on the clinical course and interpretation of laboratory tests. RESULTS: A final diagnosis of ocular toxoplasmosis was made in 88 of 155 patients (group 1, 68; group 2, 20). Among these patients, 65% had intraocular IgG production, 52% had intraocular IgA production, 37.5% had both IgG and IgA production, 27% had IgG production only, and 15% had IgA production only. Of the 13 patients tested, only one had intraocular IgM production. Intraocular IgA could not be detected in patients who had final diagnoses other than ocular toxoplasmosis or in control subjects. A positive polymerase chain reaction combined with a test that was positive for intraocular IgG production had a sensitivity of 77%, which increased to 91% after the detection of intraocular IgA production was added. CONCLUSIONS: Immunoglobulin G is the major class involved in the humoral immune response against the T. gondii parasite, followed by IgA. The determination of IgA production is useful as an additional test in the diagnosis of ocular toxoplasmosis. SN - 0002-9394 UR - https://www.unboundmedicine.com/medline/citation/10088739/Intraocular_anti_Toxoplasma_gondii_IgA_antibody_production_in_patients_with_ocular_toxoplasmosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002939498003377 DB - PRIME DP - Unbound Medicine ER -