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Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1.
Acta Crystallogr D Biol Crystallogr. 1999 Jan; 55(Pt 1):291-3.AC

Abstract

YPD1 is a yeast osmoregulatory protein that functions in a phosphorelay signal-transduction pathway. YPD1 has been expressed in Escherichia coli, purified to homogeneity and crystallized. The crystals were obtained by hanging-drop vapor-diffusion using PEG 4000 as a precipitant. Preliminary X-ray diffraction analysis indicates that the crystals belong to tetragonal space group P43212 or P41212 with unit-cell dimensions a = b = 52.71, c = 244.02 A. X-ray data to 2.7 and 3.0 A have been collected from native crystals and a heavy-atom derivative, respectively. Positions for two Hg atoms have been located by analysis of difference Patterson maps.

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Authors+Show Affiliations

Xu Q
Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA.
Nguyen V
No affiliation info available
West AH
No affiliation info available

MeSH

Base SequenceCrystallizationCrystallography, X-RayDNA PrimersDNA-Binding ProteinsEscherichia coliFungal ProteinsIntracellular Signaling Peptides and ProteinsProtein KinasesRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10089428

Citation

Xu, Q, et al. "Purification, Crystallization and Preliminary X-ray Diffraction Analysis of the Yeast Phosphorelay Protein YPD1." Acta Crystallographica. Section D, Biological Crystallography, vol. 55, no. Pt 1, 1999, pp. 291-3.
Xu Q, Nguyen V, West AH. Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):291-3.
Xu, Q., Nguyen, V., & West, A. H. (1999). Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1. Acta Crystallographica. Section D, Biological Crystallography, 55(Pt 1), 291-3.
Xu Q, Nguyen V, West AH. Purification, Crystallization and Preliminary X-ray Diffraction Analysis of the Yeast Phosphorelay Protein YPD1. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):291-3. PubMed PMID: 10089428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1. AU - Xu,Q, AU - Nguyen,V, AU - West,A H, Y1 - 1999/01/01/ PY - 1998/04/13/received PY - 1998/06/22/accepted PY - 1999/3/25/pubmed PY - 2000/6/23/medline PY - 1999/3/25/entrez SP - 291 EP - 3 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 55 IS - Pt 1 N2 - YPD1 is a yeast osmoregulatory protein that functions in a phosphorelay signal-transduction pathway. YPD1 has been expressed in Escherichia coli, purified to homogeneity and crystallized. The crystals were obtained by hanging-drop vapor-diffusion using PEG 4000 as a precipitant. Preliminary X-ray diffraction analysis indicates that the crystals belong to tetragonal space group P43212 or P41212 with unit-cell dimensions a = b = 52.71, c = 244.02 A. X-ray data to 2.7 and 3.0 A have been collected from native crystals and a heavy-atom derivative, respectively. Positions for two Hg atoms have been located by analysis of difference Patterson maps. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/10089428/Purification_crystallization_and_preliminary_X_ray_diffraction_analysis_of_the_yeast_phosphorelay_protein_YPD1_ L2 - http://scripts.iucr.org/cgi-bin/paper?S090744499800866X DB - PRIME DP - Unbound Medicine ER -